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Literature summary extracted from

  • Nam, S.J.; Kim, Y.O.; Ko, T.K.; Kang, J.K.; Chun, K.H.; Auh, J.H.; Lee, C.S.; Lee, I.K.; Park, S.; Oh, B.C.
    Molecular and biochemical characteristics of beta-propeller phytase from marine Pseudomonas sp. BS10-3 and its potential application for animal feed additives (2014), J. Microbiol. Biotechnol., 24, 1413-1420.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Ca2+ required for activity. Activation of the enzyme involves a minimum of four Ca2+-binding sites and enzyme activation is mediated by cooperative interactions between Ca2+ and the enzyme or phytate. The apparent Km increases as the Ca2+ concentration is increased, and the apparent Vmax also increases Pseudomonas sp.
3.1.3.8 Fe2+ may partly substitute for Ca2+ in activation Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 68852
-
 x * 68852, calculated Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Pseudomonas sp.
-
-
-
3.1.3.8 Pseudomonas sp. BS10-3
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + 3 H2O enzyme efficiently hydrolyzes Ca2+-phytate salts and yields myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products Pseudomonas sp. myo-inositol 2,4,6-trisphosphate + 3 phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + 3 H2O enzyme efficiently hydrolyzes Ca2+-phytate salts and yields myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products Pseudomonas sp. BS10-3 myo-inositol 2,4,6-trisphosphate + 3 phosphate
-
 ?
3.1.3.8 rice bran + H2O natural phytate source Pseudomonas sp. ? + phosphate
-
 ?
3.1.3.8 rice bran + H2O natural phytate source Pseudomonas sp. BS10-3 ? + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 ? x * 68852, calculated Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 beta-propeller phytase
-
 Pseudomonas sp.
3.1.3.8 BPP
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 55
-
 presence of 1 mM Ca2+, stable up to 55°C Pseudomonas sp.
3.1.3.8 60
-
 rapid decrease in stability above Pseudomonas sp.
3.1.3.8 70
-
 30 min, 20% residual activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 6
-
-
 Pseudomonas sp.