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Literature summary extracted from

  • Sim, S.J.; Baik, K.S.; Park, S.C.; Choe, H.N.; Seong, C.N.; Shin, T.S.; Woo, H.C.; Cho, J.Y.; Kim, D.
    Characterization of alginate lyase gene using a metagenomic library constructed from the gut microflora of abalone (2012), J. Ind. Microbiol. Biotechnol., 39, 585-593.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.3 AgNO3 activates 31% at 1 mM Klebsiella pneumoniae
4.2.2.3 CoCl2 activates 8% at 1 mM Klebsiella pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 DNA and amino acid sequence determination and analysis, lambda phage display, sequence comparisons Klebsiella pneumoniae

General Stability

EC Number General Stability Organism
4.2.2.3 AlyDW11 contains three highly conserved regions, RSEL, QIH, and YFKAGVYNQ, which may act to stabilize the three-dimensional conformation and function of the alginate lyase Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 EDTA 25% inhibition at 1 mM Klebsiella pneumoniae
4.2.2.3 FeSO4 10% inhibition at 1 mM Klebsiella pneumoniae
4.2.2.3 KCl weak inhibition at 1 mM Klebsiella pneumoniae
4.2.2.3 MgCl2 weak inhibition at 1 mM Klebsiella pneumoniae
4.2.2.3 MgSO4 weak inhibition at 1 mM Klebsiella pneumoniae
4.2.2.11 EDTA 1 mM, 75% residual activity uncultured bacterium
4.2.2.11 Mn2+ 1 mM, 85% residual activity uncultured bacterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.3 extracellular the enzyme is secreted Klebsiella pneumoniae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 additional information no or poor effects by CaCl2 and NaCl at 1 mM Klebsiella pneumoniae
4.2.2.11 Ag+ 1 mM, 131% of initial activity uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.11 77500
-
 x * 77500, SDS-PAGE of recombinant protein used to maltose binding protein uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.3 sodium alginate Klebsiella pneumoniae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Klebsiella pneumoniae Q59478 subsp. aerogenes, isolated from the gut of abalone from the South Sea near Yeosu in the Republic of Korea
-
4.2.2.11 uncultured bacterium G3LI08 isolated from gut microflora of abalone
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 poly(beta-D-manuronate) AlyDW11 prefers poly(beta-D-mannuronate) as a substrate over poly(alpha-L-guluronate) Klebsiella pneumoniae ?
-
 ?
4.2.2.3 sodium alginate
-
 Klebsiella pneumoniae ?
-
 ?
4.2.2.3 sodium alginate profiles of alginate oligomers are obtained by digestion of alginate with an alginate lyase AlyDW11, overview. The enzyme has endolytic activity and prefers poly(beta-D-mannuronate) over poly(alpha-L-guluronate) Klebsiella pneumoniae ?
-
 ?
4.2.2.11 alginate
-
 uncultured bacterium ? enzyme degrades alginate into a mixture of products with molecular masses below 1000 Da ?
4.2.2.11 poly(alpha-(1->4)-L-guluronic acid)
-
 uncultured bacterium ?
-
 ?
4.2.2.11 poly(beta-(1->4)-D-mannuronic acid) preferred over poly(alpha-(1->4)-L-guluronic acid) uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.11 ? x * 77500, SDS-PAGE of recombinant protein used to maltose binding protein uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Klebsiella pneumoniae
4.2.2.3 AlyDW11
-
 Klebsiella pneumoniae
4.2.2.3 M block-specific polymannuronate lyase
-
 Klebsiella pneumoniae
4.2.2.11 AlyDW11
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 45
-
-
 Klebsiella pneumoniae
4.2.2.11 45
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.11 30.55
-
 more than 70% of maximum activity within uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7
-
-
 Klebsiella pneumoniae
4.2.2.11 7
-
-
 uncultured bacterium