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Literature summary extracted from

  • Mizuno, T.; Shiono, Y.; Koseki, T.
    Biochemical characterization of Aspergillus oryzae native tannase and the recombinant enzyme expressed in Pichia pastoris (2014), J. Biosci. Bioeng., 118, 392-395.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.20 expressed in Pichia pastoris strain RIB40 Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 0.23
-
 epicatechin gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 0.26
-
 epigallocatechin gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 0.32
-
 epigallocatechin gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 0.34
-
 epicatechin gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 0.45
-
 catechin gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 0.47
-
 catechin gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 0.86
-
 ethyl gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 1.02
-
 methyl gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 1.12
-
 propyl gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 1.3
-
 ethyl gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 1.38
-
 propyl gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 1.97
-
 methyl gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Aspergillus oryzae
-
 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Aspergillus oryzae RIB 40
-
 gallate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus oryzae
-
-
-
3.1.1.20 Aspergillus oryzae RIB 40
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.20 glycoprotein
-
 Aspergillus oryzae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 TSKgel DEAE-5PW column chromatography and G3000-SWXL gel filtration Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 catechin gallate + H2O
-
 Aspergillus oryzae gallate + catechin
-
 ?
3.1.1.20 epicatechin gallate + H2O best substrate Aspergillus oryzae gallate + epicatechin
-
 ?
3.1.1.20 epicatechin gallate + H2O best substrate Aspergillus oryzae RIB 40 gallate + epicatechin
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Aspergillus oryzae gallate + epigallocatechin
-
 ?
3.1.1.20 ethyl gallate + H2O worst substrate Aspergillus oryzae gallate + ethanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Aspergillus oryzae gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Aspergillus oryzae RIB 40 gallate + methanol
-
 ?
3.1.1.20 additional information no activities against the methyl esters of ferulic, 4-coumaric, caffeic, and sinapic acids, or the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid Aspergillus oryzae ?
-
 ?
3.1.1.20 additional information no activities against the methyl esters of ferulic, 4-coumaric, caffeic, and sinapic acids, or the ethyl, propyl, and butyl esters of 4-hydroxybenzoic acid Aspergillus oryzae RIB 40 ?
-
 ?
3.1.1.20 propyl gallate + H2O
-
 Aspergillus oryzae gallate + propanol
-
 ?
3.1.1.20 propyl gallate + H2O
-
 Aspergillus oryzae RIB 40 gallate + propanol
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus oryzae gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus oryzae RIB 40 gallate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 ? x * 30000-33000, deglycosylated native enzyme, SDS-PAGE Aspergillus oryzae
3.1.1.20 ? x * 30000-34000, deglycosylated recombinant enzyme, SDS-PAGE Aspergillus oryzae
3.1.1.20 ? x * 45000-75000, recombinant enzyme, smeared bands on SDS-PAGE Aspergillus oryzae
3.1.1.20 ? x * 45000-80000, native enzyme, smeared bands on SDS-PAGE Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 TanA
-
 Aspergillus oryzae
3.1.1.20 tannin acyl hydrolase
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 40 50 the enzyme is stable up to 40°C. Native enzyme retains more than 70% of ist activity after incubation at 50°C for 60 min, whereas the residual activity of recombinant enzyme is approximately 40% after treatment at 50°C for 60 min Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.20 9.8
-
 ethyl gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 18.6
-
 propyl gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 19
-
 ethyl gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 20.5
-
 propyl gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 60.2
-
 methyl gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 62.1
-
 epigallocatechin gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 64.5
-
 catechin gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 67.5
-
 epicatechin gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 70.2
-
 methyl gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 79.8
-
 catechin gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 86.1
-
 epigallocatechin gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 103.4
-
 epicatechin gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 4 5 native enzyme Aspergillus oryzae
3.1.1.20 5
-
 purified enzyme Aspergillus oryzae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.20 7.54
-
 ethyl gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 14.9
-
 propyl gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 16.6
-
 propyl gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 22.1
-
 ethyl gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 30.6
-
 methyl gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 69.2
-
 methyl gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 137.3
-
 catechin gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 179.3
-
 catechin gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 234
-
 epigallocatechin gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 273.4
-
 epigallocatechin gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 293.6
-
 epicatechin gallate recombinant enzyme, at pH 5.0 and 30°C Aspergillus oryzae
3.1.1.20 308.8
-
 epicatechin gallate native enzyme, at pH 5.0 and 30°C Aspergillus oryzae