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Literature summary extracted from

  • Hoffmann, I.; Jerneren, F.; Oliw, E.H.
    Expression of fusion proteins of Aspergillus terreus reveals a novel allene oxide synthase (2013), J. Biol. Chem., 288, 11459-11469.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.92 gene ATEG_02036, sequence comparisons with other enzymes, cloning and functional expression of the enzyme with V5 epitope and His6-tag from plasmid pIZ/V5-His in Spodoptera frugiperda Sf9 cells and in Escherichia coli Aspergillus terreus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.92 C1073S site-directed mutagenesis, the substitution in the P450 domain abolishes allene oxide synthase activity Aspergillus terreus
4.2.1.92 N964D site-directed mutagenesis, the mutation markedly reduces allene oxide synthase activity Aspergillus terreus
4.2.1.92 N964V site-directed mutagenesis, the mutation markedly reduces allene oxide synthase activity Aspergillus terreus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.92 membrane
-
 Aspergillus terreus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.92 133000
-
 recombinant C-terminally His6-tagged, V5-tagged enzyme Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.92 (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Aspergillus terreus
-
 (9R,10)-10-epoxy-(10Z,12Z)-octadecadienoic acid an unstable allene oxide ?
4.2.1.92 (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Aspergillus terreus A1156
-
 (9R,10)-10-epoxy-(10Z,12Z)-octadecadienoic acid an unstable allene oxide ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.92 Aspergillus terreus Q0CW98 gene ATEG_02036; gene ATEG_02036
-
4.2.1.92 Aspergillus terreus A1156 Q0CW98 gene ATEG_02036; gene ATEG_02036
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.92 mycelium
-
 Aspergillus terreus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.92 (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Aspergillus terreus (9R,10)-10-epoxy-(10Z,12Z)-octadecadienoic acid an unstable allene oxide ?
4.2.1.92 (9R,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Aspergillus terreus A1156 (9R,10)-10-epoxy-(10Z,12Z)-octadecadienoic acid an unstable allene oxide ?
4.2.1.92 additional information Asn964 may facilitate homolytic cleavage of the dioxygen bond of (9R)-hydroperoxy-10(E),12(Z)-octadecadienoic acid with formation of compound II. No activity with 18:2n-6 Aspergillus terreus ?
-
 ?
4.2.1.92 additional information Asn964 may facilitate homolytic cleavage of the dioxygen bond of (9R)-hydroperoxy-10(E),12(Z)-octadecadienoic acid with formation of compound II. No activity with 18:2n-6 Aspergillus terreus A1156 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.92 9R-DOX-AOS
-
 Aspergillus terreus
4.2.1.92 allene oxide synthase
-
 Aspergillus terreus
4.2.1.92 AOS
-
 Aspergillus terreus
4.2.1.92 KC525886
-
 Aspergillus terreus

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.92 cytochrome P450 6003B Aspergillus terreus

General Information

EC Number General Information Comment Organism
4.2.1.92 evolution the fungal allene oxide synthases belongs to the CYP74 family Aspergillus terreus