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Literature summary extracted from

  • Martinez, V.; Ingwers, M.; Smith, J.; Glushka, J.; Yang, T.; Bar-Peled, M.
    Biosynthesis of UDP-4-keto-6-deoxyglucose and UDP-rhamnose in pathogenic fungi Magnaporthe grisea and Botryotinia fuckeliana (2012), J. Biol. Chem., 287, 879-892.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.76 expressed in Escherichia coli BL21 cells Botrytis cinerea
4.2.1.76 expressed in Escherichia coli BL21 cells Pyricularia grisea

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.76 additional information not inhibited by NADH Botrytis cinerea
4.2.1.76 additional information not inhibited by NADH Pyricularia grisea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.76 52000
-
 3 * 52000, SDS-PAGE Pyricularia grisea
4.2.1.76 52500
-
 3 * 52500, SDS-PAGE Botrytis cinerea
4.2.1.76 140000
-
 gel filtration Botrytis cinerea
4.2.1.76 140000
-
 gel filtration Pyricularia grisea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.76 UDP-glucose Botrytis cinerea
-
 UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 UDP-glucose Pyricularia grisea
-
 UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 UDP-glucose Pyricularia grisea CP987
-
 UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 UDP-glucose Botrytis cinerea B05.10
-
 UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.76 Botrytis cinerea F8U971
-
-
4.2.1.76 Botrytis cinerea B05.10 F8U971
-
-
4.2.1.76 Pyricularia grisea F8U969
-
-
4.2.1.76 Pyricularia grisea CP987 F8U969
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.76 nickel-Sepharose column chromatography Botrytis cinerea
4.2.1.76 nickel-Sepharose column chromatography Pyricularia grisea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.76 dTDP-glucose this enzymatic reaction is much less efficient compared to UDP-glucose Botrytis cinerea dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 dTDP-glucose this enzymatic reaction is much less efficient compared to UDP-glucose Pyricularia grisea dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 dTDP-glucose this enzymatic reaction is much less efficient compared to UDP-glucose Pyricularia grisea CP987 dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 dTDP-glucose this enzymatic reaction is much less efficient compared to UDP-glucose Botrytis cinerea B05.10 dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 UDP-glucose
-
 Botrytis cinerea UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 UDP-glucose
-
 Pyricularia grisea UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 UDP-glucose
-
 Pyricularia grisea CP987 UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.76 UDP-glucose
-
 Botrytis cinerea B05.10 UDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.76 homotrimer 3 * 52500, SDS-PAGE Botrytis cinerea
4.2.1.76 homotrimer 3 * 52000, SDS-PAGE Pyricularia grisea

Synonyms

EC Number Synonyms Comment Organism
4.2.1.76 UDP-glucose-4,6-dehydratase
-
 Botrytis cinerea
4.2.1.76 UDP-glucose-4,6-dehydratase
-
 Pyricularia grisea
4.2.1.76 UG4,6-Dh
-
 Botrytis cinerea
4.2.1.76 UG4,6-Dh
-
 Pyricularia grisea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.76 25 30
-
 Botrytis cinerea
4.2.1.76 25 30
-
 Pyricularia grisea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.76 7
-
-
 Botrytis cinerea
4.2.1.76 7
-
-
 Pyricularia grisea

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.76 NAD+ required Botrytis cinerea
4.2.1.76 NAD+ required Pyricularia grisea