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Literature summary extracted from

  • Xie, X.; Meehan, M.J.; Xu, W.; Dorrestein, P.C.; Tang, Y.
    Acyltransferase mediated polyketide release from a fungal megasynthase (2009), J. Am. Chem. Soc., 131, 8388-8389.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.238 S76A active site mutant, no activity Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.238 0.039
-
 (S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase] pH and temperature not specified in the publication Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.238 monacolin J acid + (S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase] Aspergillus terreus the enzyme is involved in lovastatin biosynthesis lovastatin acid + [2-methylbutanoate polyketide synthase]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.238 Aspergillus terreus Q9Y7D1
-
-
2.3.1.244 Aspergillus terreus Q9Y7D5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.238 monacolin J acid + (S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase] the enzyme is involved in lovastatin biosynthesis Aspergillus terreus lovastatin acid + [2-methylbutanoate polyketide synthase]
-
 ?
2.3.1.238 monacolin J acid + (S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase] the enzyme primarily interacts with the ACP domain of 2-methylbutanoate polyketide synthase (LovF) and the protein-protein interactions leads to highly efficient transfer of the diketide product Aspergillus terreus lovastatin acid + [2-methylbutanoate polyketide synthase]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.238 LovD
-
 Aspergillus terreus
2.3.1.244 lovastatin diketide synthase
-
 Aspergillus terreus
2.3.1.244 LovF
-
 Aspergillus terreus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.238 0.806
-
 (S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase] pH and temperature not specified in the publication Aspergillus terreus

General Information

EC Number General Information Comment Organism
2.3.1.238 physiological function the enzyme is involved in lovastatin biosynthesis Aspergillus terreus
2.3.1.244 physiological function enzyme LovF synthesizes the alpha-S-methylbutyrate side chain that is subsequently transferred to monacolin J to yield the cholesterol-lowering natural product lovastatin. The diketide product of LovF is offloaded from the LovF acyl carrier protein domain by the dissociated acyltransferase LovD. LovD primarily interacts with the acyl carrier protein domain of LovF and the protein-protein interactions lead to highly efficient transfer of the diketide product. The catalytic efficiency is enhanced nearly one millionfold when LovF is used as the acyl carrier instead of N-acetylcysteamine Aspergillus terreus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.238 20.7
-
 (S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase] pH and temperature not specified in the publication Aspergillus terreus