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Literature summary extracted from
- Characterization and application of calcium-dependent ?-propeller phytase from Bacillus amyloliquefaciens DS11 (2012), J. Agric. Food Chem., 60, 7532-7537.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | D308A | 50% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | D308E | 94% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | D56A | 0.4% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | D56E | 20% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | D56L | 0.6% of wild-type activity, significant decrease in melting temperature and optimum temperature | Bacillus sp. (in: Bacteria) |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Ca2+ | - |
Bacillus sp. (in: Bacteria) |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 0.1363 | - |
myo-inositol hexakisphosphate | wild-type, pH 9.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.138 | - |
myo-inositol hexakisphosphate | wild-type, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.143 | - |
myo-inositol hexakisphosphate | wild-type, pH 8.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.146 | - |
myo-inositol hexakisphosphate | wild-type, pH 6.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.151 | - |
myo-inositol hexakisphosphate | wild-type, pH 5.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.156 | - |
myo-inositol hexakisphosphate | mutant D308E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.159 | - |
myo-inositol hexakisphosphate | wild-type, pH 4.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.167 | - |
myo-inositol hexakisphosphate | mutant D308A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.189 | - |
myo-inositol hexakisphosphate | mutant D56E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.218 | - |
myo-inositol hexakisphosphate | mutant D56L, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.248 | - |
myo-inositol hexakisphosphate | mutant D56A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Ca2+ | enzyme requires excess Ca2+ for its enzymatic activity as the pH decreases | Bacillus sp. (in: Bacteria) | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Bacillus sp. (in: Bacteria) | O66037 | - |
- |
| 3.1.3.8 | Bacillus sp. (in: Bacteria) DS11 | O66037 | - |
- |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | enzyme can restore its enzymatic activity in the presence of 5 mM Ca2+ to 87% of its original activity after heating to 100°C and subsequent cooling | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Bacillus sp. (in: Bacteria) | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Bacillus sp. (in: Bacteria) DS11 | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | beta-propeller phytase | - |
Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | BPP | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 50 | - |
mutants D56L, D308A | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | 55 | - |
mutant D308E | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | 60 | - |
mutant D56E | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | 80 | - |
wild-type | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 48.6 | - |
mutant D308A, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | 48.8 | - |
wild-type, melting temperature, presence of EDTA, mutant D56A, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | 49.6 | - |
mutant D56L, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | 54.8 | - |
mutant D308E, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | 55.5 | - |
mutant D56E, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
| 3.1.3.8 | 80 | - |
wild-type, melting temperature, presence of 5 mM Ca2+ | Bacillus sp. (in: Bacteria) |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 0.07 | - |
myo-inositol hexakisphosphate | mutant D56A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.1 | - |
myo-inositol hexakisphosphate | mutant D56L, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 3.34 | - |
myo-inositol hexakisphosphate | mutant D56E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 8.33 | - |
myo-inositol hexakisphosphate | mutant D308A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 15.55 | - |
myo-inositol hexakisphosphate | mutant D308E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 16.55 | - |
myo-inositol hexakisphosphate | wild-type, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 4.5 | - |
Ca2+ | wild-type, pH 9.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 5.2 | - |
Ca2+ | wild-type, pH 8.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 7.4 | - |
Ca2+ | wild-type, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 17.6 | - |
Ca2+ | wild-type, pH 6.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 43.6 | - |
Ca2+ | wild-type, pH 5.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 738 | - |
Ca2+ | wild-type, pH 4.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 0.27 | - |
myo-inositol hexakisphosphate | mutant D56A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 0.44 | - |
myo-inositol hexakisphosphate | mutant D56E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 17.67 | - |
myo-inositol hexakisphosphate | mutant D56L, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 49.9 | - |
myo-inositol hexakisphosphate | mutant D308A, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 99.7 | - |
myo-inositol hexakisphosphate | mutant D308E, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
| 3.1.3.8 | 119.9 | - |
myo-inositol hexakisphosphate | wild-type, pH 7.0, temperature not specified in the publication | Bacillus sp. (in: Bacteria) | |
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