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Literature summary extracted from

  • Srinivasan, B.; Forouhar, F.; Shukla, A.; Sampangi, C.; Kulkarni, S.; Abashidze, M.; Seetharaman, J.; Lew, S.; Mao, L.; Acton, T.B.; Xiao, R.; Everett, J.K.; Montelione, G.T.; Tong, L.; Balaram, H.
    Allosteric regulation and substrate activation in cytosolic nucleotidase II from Legionella pneumophila (2014), FEBS J., 281, 1613-1628.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.5 dGTP heterotrophic allosteric activator Legionella pneumophila
3.1.3.5 GDP heterotrophic allosteric activator Legionella pneumophila
3.1.3.5 GMP substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot Legionella pneumophila
3.1.3.5 GTP heterotrophic allosteric activator Legionella pneumophila
3.1.3.5 Mg2+ preferred divalent cation Legionella pneumophila
3.1.3.5 Mn2+ may substitute for Mg2+ as divalent cation Legionella pneumophila

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.5 in complex with phosphate and GMP, to 2.7 A nd 2.5 A resolution, respectively. Structures reveal an activator-binding site at the dimer interface Legionella pneumophila

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.5 R86A same level of basal activity as the wild-type for the substrate 4-nitrophenyl phosphate, but activation of 4-nitrophenyl phosphate hydrolysis by GTP is compromised Legionella pneumophila
3.1.3.5 Y421S same level of basal activity as the wild-type for the substrate 4-nitrophenyl phosphate, but activation of 4-nitrophenyl phosphate hydrolysis by GTP is compromised Legionella pneumophila
3.1.3.5 Y421S/R86A same level of basal activity as the wild-type for the substrate 4-nitrophenyl phosphate, but activation of 4-nitrophenyl phosphate hydrolysis by GTP is abolished Legionella pneumophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 7.2
-
 5'-GMP sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila
3.1.3.5 18
-
 5'-IMP biphasic kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila
3.1.3.5 142
-
 5'-dGMP sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Legionella pneumophila Q5ZZB6
-
-
3.1.3.5 Legionella pneumophila DSM 7513 Q5ZZB6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-dGMP + H2O
-
 Legionella pneumophila deoxyguanosine + phosphate
-
 ?
3.1.3.5 5'-dGMP + H2O
-
 Legionella pneumophila DSM 7513 deoxyguanosine + phosphate
-
 ?
3.1.3.5 5'-dIMP + H2O
-
 Legionella pneumophila deoxyinosine + phosphate
-
 ?
3.1.3.5 5'-dIMP + H2O
-
 Legionella pneumophila DSM 7513 deoxyinosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot Legionella pneumophila guanosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O preferred substrate. Substrate GMP acting as an activator, partitioning between the allosteric and active site, is the basis for the sigmoidicity of the initial velocity versus GMP concentration plot Legionella pneumophila DSM 7513 guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Legionella pneumophila inosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O
-
 Legionella pneumophila DSM 7513 inosine + phosphate
-
 ?
3.1.3.5 additional information activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor Legionella pneumophila ?
-
 ?
3.1.3.5 additional information activity on AMP, XMP, CMP, UMP and -nitrophenyl phosphate is poor Legionella pneumophila DSM 7513 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 cN-II
-
 Legionella pneumophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.5 0.3
-
 5'-IMP pH 8.0, temperature not specified in the publication Legionella pneumophila
3.1.3.5 12.4
-
 5'-GMP pH 8.0, temperature not specified in the publication Legionella pneumophila
3.1.3.5 20.7
-
 5'-dGMP pH 8.0, temperature not specified in the publication Legionella pneumophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.5 0.02
-
 5'-IMP biphasic kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila
3.1.3.5 0.15
-
 5'-dGMP sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila
3.1.3.5 1.7
-
 5'-GMP sigmoidal kinetic profile, pH 8.0, temperature not specified in the publication Legionella pneumophila