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Literature summary extracted from

  • Zhang, Q.F.; Gu, J.; Gong, P.; Wang, X.D.; Tu, S.; Bi, L.J.; Yu, Z.N.; Zhang, Z.P.; Cui, Z.Q.; Wei, H.P.; Tao, S.C.; Zhang, X.E.; Deng, J.Y.
    Reversibly acetylated lysine residues play important roles in the enzymatic activity of Escherichia coli N-hydroxyarylamine O-acetyltransferase (2013), FEBS J., 280, 1966-1979.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.B43
-
 Escherichia coli
2.3.1.118 expressed in Escherichia coli AD494 and BL21(DE3) cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.118 G127A mutation has no obvious effect on enzymatic activity Escherichia coli
2.3.1.118 K214R the mutant exhibits an about 25% decrease in enzyme activity compared to the wild type enzyme Escherichia coli
2.3.1.118 K214R/K281R the mutant exhibits an about 25% decrease in enzyme activity compared to the wild type enzyme Escherichia coli
2.3.1.118 K281R the mutant exhibits an about 12% decrease in enzyme activity compared to the wild type enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.B43 NAD+ + [N-hydroxyarylamine O-acetyltransferase]-N6-acetyl-L-lysine Escherichia coli
-
 nicotinamide + [N-hydroxyarylamine O-acetyltransferase]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + [N-hydroxyarylamine O-acetyltransferase]-N6-acetyl-L-lysine Escherichia coli AD494
-
 nicotinamide + [N-hydroxyarylamine O-acetyltransferase]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B43 Escherichia coli
-
-
-
2.3.1.B43 Escherichia coli AD494
-
-
-
2.3.1.118 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.B43
-
 Escherichia coli
2.3.1.118 Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B43 additional information global identification of CobB substrates using an Escherichia coli proteome microarray Escherichia coli ?
-
 ?
2.3.1.B43 additional information global identification of CobB substrates using an Escherichia coli proteome microarray Escherichia coli AD494 ?
-
 ?
2.3.1.B43 NAD+ + [N-hydroxyarylamine O-acetyltransferase]-N6-acetyl-L-lysine
-
 Escherichia coli nicotinamide + [N-hydroxyarylamine O-acetyltransferase]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.B43 NAD+ + [N-hydroxyarylamine O-acetyltransferase]-N6-acetyl-L-lysine
-
 Escherichia coli AD494 nicotinamide + [N-hydroxyarylamine O-acetyltransferase]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
2.3.1.118 acetyl-CoA + N-hydroxy-2-aminofluorene
-
 Escherichia coli CoA + N-acetyloxy-2-aminofluorene
-
 ?
2.3.1.118 acetyl-CoA + N-hydroxy-2-aminofluorene
-
 Escherichia coli AD494 CoA + N-acetyloxy-2-aminofluorene
-
 ?
2.3.1.118 additional information the enzyme is deacetylated by CobB Escherichia coli ?
-
 ?
2.3.1.118 additional information the enzyme is deacetylated by CobB Escherichia coli AD494 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.B43 CobB
-
 Escherichia coli
2.3.1.118 N-hydroxyarylamine O-acetyltransferase
-
 Escherichia coli
2.3.1.118 NhoA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.B43 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.B43 8
-
 assay at Escherichia coli