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Literature summary extracted from

  • Rombola, T.; Pedrinho, E.; De Macedo Lemos, E.; Gonsalves, A.; Dos Santos, L.; Pizauro, J.
    Identification and enzymatic characterization of acid phosphatase from Burkholderia gladioli (2014), BMC Res. Notes, 7, 221.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 4-hydroxymercuribenzoate 1 mM, 5% residual activity Burkholderia gladioli
3.1.3.2 arsenate 1 mM, 14% residual activity Burkholderia gladioli
3.1.3.2 additional information no inhibition by excess of substrate is observed. Not inhibitory: calcium, levamisole, sodium tartrate, EDTA, zinc, magnesium, cobalt, ouabain, oligomycin or pantoprazol Burkholderia gladioli
3.1.3.2 phosphate 10 mM, 38% residual activity Burkholderia gladioli
3.1.3.2 vanadate 0.5 mM, 6% residual activity Burkholderia gladioli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 additional information
-
 additional information enzyme displays cooperative kinetics with n = 1.3, Vm = 113.5 U/mg and K0.5 = 65 microM Burkholderia gladioli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 membrane
-
 Burkholderia gladioli 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Burkholderia gladioli
-
-
-
3.1.3.2 Burkholderia gladioli R 3.25
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 103.9
-
 pH 6.0, 37°C Burkholderia gladioli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate
-
 Burkholderia gladioli 4-nitrophenol + phosphate
-
 ?
3.1.3.2 4-nitrophenyl phosphate
-
 Burkholderia gladioli R 3.25 4-nitrophenol + phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 45
-
 membrane-bound enzyme, pH 6.0, stable for 6 h Burkholderia gladioli
3.1.3.2 50
-
 half-life 23 h Burkholderia gladioli
3.1.3.2 70
-
 half-life 5 min Burkholderia gladioli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.2 6
-
-
 Burkholderia gladioli

Expression

EC Number Organism Comment Expression
3.1.3.2 Burkholderia gladioli expression is strictly regulated, optimal expression at a concentration of 5 mM phosphate additional information