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Literature summary extracted from

  • Kumar, C.G.; Kamle, A.; Kamal, A.
    Purification and biochemical characterization of feruloyl esterases from Aspergillus terreus MTCC 11096 (2013), Biotechnol. Prog., 29, 924-932.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 Cu2+
-
 Aspergillus terreus
3.1.1.73 Fe2+
-
 Aspergillus terreus
3.1.1.73 Fe3+
-
 Aspergillus terreus
3.1.1.73 Hg2+ complete inhibition at 1 mM; complete inhibition at 1 mM Aspergillus terreus
3.1.1.73 K+
-
 Aspergillus terreus
3.1.1.73 Mg2+
-
 Aspergillus terreus
3.1.1.73 PMSF complete inhibition at 1 mM; complete inhibition at 1 mM Aspergillus terreus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 additional information Ca2+ has no significant effect on the enzyme activity Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 23000
-
 x * 23000, SDS-PAGE Aspergillus terreus
3.1.1.73 36000
-
 x * 36000, SDS-PAGE Aspergillus terreus
3.1.1.73 76000
-
 x * 76000, SDS-PAGE Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus terreus
-
 isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-2
-
3.1.1.73 Aspergillus terreus
-
 isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-3
-
3.1.1.73 Aspergillus terreus Q0CBM7 isolated from the soils of agricultural fields cultivating sweet sorghum, type A isozyme AtFAE-1
-
3.1.1.73 Aspergillus terreus MTCC 11096
-
 isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-2
-
3.1.1.73 Aspergillus terreus MTCC 11096
-
 isolated from the soils of agricultural fields cultivating sweet sorghum, type C isozyme AtFAE-3
-
3.1.1.73 Aspergillus terreus MTCC 11096 Q0CBM7 isolated from the soils of agricultural fields cultivating sweet sorghum, type A isozyme AtFAE-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 native isozyme AtFAE-1 67fold from strain MTCC 11096 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography Aspergillus terreus
3.1.1.73 native isozyme AtFAE-2 58fold from strain MTCC 11096 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration Aspergillus terreus
3.1.1.73 native isozyme AtFAE-3 56fold from strain MTCC 11096 by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration Aspergillus terreus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 263.3
-
 purified isozyme, pH 5.0, 40°C Aspergillus terreus
3.1.1.73 275.5
-
 purified isozyme, pH 5.0, 40°C Aspergillus terreus
3.1.1.73 317.7
-
 purified isozyme, pH 5.0, 40°C Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus terreus methanol + ferulate
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus terreus MTCC 11096 methanol + ferulate
-
 ?
3.1.1.73 additional information release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-1 for diverse ester substrates, overview Aspergillus terreus ?
-
 ?
3.1.1.73 additional information release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-2 for diverse ester substrates, overview Aspergillus terreus ?
-
 ?
3.1.1.73 additional information release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-3 for diverse ester substrates, overview Aspergillus terreus ?
-
 ?
3.1.1.73 additional information release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-2 for diverse ester substrates, overview Aspergillus terreus MTCC 11096 ?
-
 ?
3.1.1.73 additional information release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-3 for diverse ester substrates, overview Aspergillus terreus MTCC 11096 ?
-
 ?
3.1.1.73 additional information release of ferulic acid from destarched maize bran, substrate specificity of isozyme AtFAE-1 for diverse ester substrates, overview Aspergillus terreus MTCC 11096 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 76000, SDS-PAGE Aspergillus terreus
3.1.1.73 ? x * 36000, SDS-PAGE Aspergillus terreus
3.1.1.73 ? x * 23000, SDS-PAGE Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 AtFAE-1
-
 Aspergillus terreus
3.1.1.73 AtFAE-2
-
 Aspergillus terreus
3.1.1.73 AtFAE-3
-
 Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
-
 Aspergillus terreus
3.1.1.73 50
-
-
 Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 40
-
 purified isozyme AtFAE-2, 4 h incubation at pH 3.0-8.0, over 50% activity remaining Aspergillus terreus
3.1.1.73 40
-
 purified isozyme AtFAE-3, 4 h incubation at pH 3.0-8.0, over 50% activity remaining Aspergillus terreus
3.1.1.73 50
-
 purified isozyme AtFAE-1, 4 h incubation at pH 3.0-8.0, over 50% activity remaining Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
-
 Aspergillus terreus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 5 8 over 50% of maximal activity within this range Aspergillus terreus
3.1.1.73 5 6 over 50% of maximal activity within this range Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 3 8 purified isozyme AtFAE-1, 4 h incubation at 50°C, over 50% activity remaining. After 12 h incubation, the enzyme is more stable in acidic to neutral conditions than in alkaline conditions Aspergillus terreus
3.1.1.73 3 8 purified isozyme AtFAE-2, 4 h incubation at 40°C, over 50% activity remaining. After 12 h incubation, the enzyme is more stable in acidic to neutral conditions than in alkaline conditions Aspergillus terreus
3.1.1.73 3 8 purified isozyme AtFAE-3, 4 h incubation at 50°C, over 50% activity remaining. After 12 h incubation, the enzyme is more stable in acidic to neutral conditions than in alkaline conditions Aspergillus terreus