EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.4 | gene pld, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Streptomyces antibioticus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.4 | D40H/T291Y | site-directed mutagenesis of residues D40 and T291 located within dynamic surface loops, the mutant is able to synthesize phosphatidylinositol by transphosphatidylation | Streptomyces antibioticus |
3.1.4.4 | W187D/Y191Y/Y385R | site-directed mutagenesis, the mutant is able to synthesize phosphatidylinositol by transphosphatidylation, thermostabilization of enzyme mutant W187D/Y191Y/Y385R, termed DYR, is attempted by rational design based on deletion of the D40 loop, generating two variants, DELTA37-45 DYR and DELTA38-46 DYR PLD. DELTA38-46 DYR shows highest thermostability as its activity half-life at 70°C proves 11.7 and 8.0 times longer than that of the DYR mutant and mutant DELTA37-45 DYR, respectively, molecular dynamics, overview | Streptomyces antibioticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.4 | phosphatidylcholine + H2O | Streptomyces antibioticus | - |
choline + phosphatidate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.4 | Streptomyces antibioticus | Q53728 | gene pld | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.4 | phosphatidylcholine + H2O | - |
Streptomyces antibioticus | choline + phosphatidate | - |
? | |
3.1.4.4 | phosphatidylcholine + myo-inositol | the wild-type enzyme is capable of synthesizing phosphatidylinositol by transphosphatidylation. Increase in phosphatidylinositol yield is possible by providing excess of solvated myo-inositol, which is achievable at high temperatures due to its highly temperature-dependent solubility, especially by enzyme variants with increased thermostability, e.g. mutant W187D/Y191Y/Y385R | Streptomyces antibioticus | phosphatidylinositol + choline | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.4 | PLD | - |
Streptomyces antibioticus |
3.1.4.4 | SaPLD | - |
Streptomyces antibioticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | 30 | - |
assay at | Streptomyces antibioticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | 5.6 | - |
assay at | Streptomyces antibioticus |