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Literature summary extracted from

  • Tauzin, A.S.; Sulzenbacher, G.; Lafond, M.; Desseaux, V.; Reca, I.B.; Perrier, J.; Bellincampi, D.; Fourquet, P.; Leveque, C.; Giardina, T.
    Functional characterization of a vacuolar invertase from Solanum lycopersicum: post-translational regulation by N-glycosylation and a proteinaceous inhibitor (2014), Biochimie, 101, 39-49.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26 expressed in Pichia pastoris Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.26 N119D the mutant shows reduced activity compared to the wild type enzyme Solanum lycopersicum
3.2.1.26 N184D the mutant shows reduced activity compared to the wild type enzyme Solanum lycopersicum
3.2.1.26 N516D the mutation dramatically affects the folding of the protein. The mutant is inactive Solanum lycopersicum
3.2.1.26 N52D the mutant shows reduced activity compared to the wild type enzyme Solanum lycopersicum
3.2.1.26 N52D/N119D/N184D the mutant shows reduced activity compared to the wild type enzyme Solanum lycopersicum
3.2.1.26 N52D/N119D/N184D/N516D the mutations dramatically affect the folding of the protein. The mutant is inactive Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 SolyVIF non-competitive inhibition Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 14
-
 sucrose at pH 4.5 and 50°C Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 vacuole
-
 Solanum lycopersicum 5773
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 60566
-
 x * 60566, calculated from amino acid sequence Solanum lycopersicum
3.2.1.26 69114
-
 x * 69114, mass spectrometry Solanum lycopersicum
3.2.1.26 70000
-
 x * 70000, deglycosylated protein, SDS-PAGE Solanum lycopersicum
3.2.1.26 79000
-
 x * 79000, glycoprotein, SDS-PAGE Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Solanum lycopersicum P29000 cultivar Red Moneymaker
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.26 glycoprotein the enzyme has four N-glycosylation sites. Glycosylation of N516 is crucial for recombinant enzyme stability Solanum lycopersicum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 ammonium sulfate precipitation and Sephacryl S-200 gel filtration Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 flower highest expression Solanum lycopersicum
-
3.2.1.26 fruit high expression in mature fruit Solanum lycopersicum
-
3.2.1.26 root
-
 Solanum lycopersicum
-
3.2.1.26 stem
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 sucrose + H2O
-
 Solanum lycopersicum fructose + glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 ? x * 60566, calculated from amino acid sequence Solanum lycopersicum
3.2.1.26 ? x * 69114, mass spectrometry Solanum lycopersicum
3.2.1.26 ? x * 70000, deglycosylated protein, SDS-PAGE Solanum lycopersicum
3.2.1.26 ? x * 79000, glycoprotein, SDS-PAGE Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 TIV-1
-
 Solanum lycopersicum
3.2.1.26 Vacuolar invertase
-
 Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.26 1384
-
 sucrose at pH 4.5 and 50°C Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 4.5
-
-
 Solanum lycopersicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.26 99
-
 sucrose at pH 4.5 and 50°C Solanum lycopersicum