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Literature summary extracted from

  • Light, S.H.; Antanasijevic, A.; Krishna, S.N.; Caffrey, M.; Anderson, W.F.; Lavie, A.
    Crystal structures of type I dehydroquinate dehydratase in complex with quinate and shikimate suggest a novel mechanism of Schiff base formation (2014), Biochemistry, 53, 872-880.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.10 expressed in Escherichia coli BL21(DE3) cells Salmonella enterica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.10 in complex with quinate and shikimate, sitting drop vapor diffusion method, using 0.01 M nickel chloride, 0.1 M Tris (pH 8.5), and 20% poly(ethylene glycol) monomethyl ether 2000 Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.10 K170M the mutant shows enhanced affinity towards quinate and shikimate Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.10 3-dehydroquinate Salmonella enterica
-
 3-dehydroshikimate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.10 Salmonella enterica P58687
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.10 Ni-NTA column chromatography Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.10 3-dehydroquinate
-
 Salmonella enterica 3-dehydroshikimate + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.10 DHQD
-
 Salmonella enterica
4.2.1.10 type I dehydroquinate dehydratase
-
 Salmonella enterica