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Literature summary extracted from

  • Coderch, C.; Lence, E.; Peon, A.; Lamb, H.; Hawkins, A.R.; Gago, F.; Gonzalez-Bello, C.
    Mechanistic insight into the reaction catalysed by bacterial type II dehydroquinases (2014), Biochem. J., 458, 547-557.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.10 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
4.2.1.10 expressed in Escherichia coli BL21(DE3) cells Helicobacter pylori

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.10 D88N the mutant shows severely reduced activity compared to the wild type enzyme and is not active at pH 7.0 but pH 8.0 Mycobacterium tuberculosis
4.2.1.10 N12S the mutant shows severely reduced activity compared to the wild type enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.10 0.0114
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme N12S, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis
4.2.1.10 0.0146
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid wild type enzyme, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis
4.2.1.10 0.1776
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme D88N, in Tris/acetic acid (50 mM, pH 8.0) at 25°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.10 3-dehydroquinate Mycobacterium tuberculosis
-
 3-dehydroshikimate + H2O
-
 ?
4.2.1.10 3-dehydroquinate Helicobacter pylori
-
 3-dehydroshikimate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.10 Helicobacter pylori
-
-
-
4.2.1.10 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.10 Q Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography Mycobacterium tuberculosis
4.2.1.10 Q Sepharose column chromatography, hydroxyapatite column chromatography, and Mono Q column chromatography Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.10 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid
-
 Mycobacterium tuberculosis ?
-
 ?
4.2.1.10 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid
-
 Helicobacter pylori ?
-
 ?
4.2.1.10 3-dehydroquinate
-
 Mycobacterium tuberculosis 3-dehydroshikimate + H2O
-
 ?
4.2.1.10 3-dehydroquinate
-
 Helicobacter pylori 3-dehydroshikimate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.10 DHQ2
-
 Mycobacterium tuberculosis
4.2.1.10 DHQ2
-
 Helicobacter pylori
4.2.1.10 Type II dehydroquinase
-
 Mycobacterium tuberculosis
4.2.1.10 Type II dehydroquinase
-
 Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.10 0.0006
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme D88N, in Tris/acetic acid (50 mM, pH 8.0) at 25°C Mycobacterium tuberculosis
4.2.1.10 0.0065
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme N12S, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis
4.2.1.10 0.1542
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid wild type enzyme, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.10 7
-
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.10 0.03
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme D88N, in Tris/acetic acid (50 mM, pH 8.0) at 25°C Mycobacterium tuberculosis
4.2.1.10 0.6
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid mutant enzyme N12S, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis
4.2.1.10 10.5
-
 (2R)-2-(4-methoxybenzyl)-3-dehydroquinic acid wild type enzyme, in Tris/acetic acid (50 mM, pH 7.0) at 25°C Mycobacterium tuberculosis