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Literature summary extracted from

  • Schlieben, N.H.; Niefind, K.; Schomburg, D.
    Expression, purification, and aggregation studies of His-tagged thermoalkalophilic lipase from Bacillus thermocatenulatus (2004), Protein Expr. Purif., 34, 103-110.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 His-tagged enzyme is expressed in Escherichia coli Geobacillus thermocatenulatus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.3 vapour-diffusion method at 20°C. The purified BTL2 lipase shows a strong tendency to aggregate upon concentration, which prevents a reproducible crystallization. Aggregation studies using dynamic light-scattering analysis are performed to improve the purification and concentration of BTL2 lipase. Different chemical classes of additives are tested to manipulate the aggregation behaviour of BTL2 lipase with the aim of obtaining a monodisperse sample to use for crystallization Geobacillus thermocatenulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 40000
-
 x * 40000, SDS-PAGE Geobacillus thermocatenulatus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 2-propanol for the concentrated lipase, the availability of 5% 2-propanol is sufficient to hold the lipase in monomeric form Geobacillus thermocatenulatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Geobacillus thermocatenulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3
-
 Geobacillus thermocatenulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Geobacillus thermocatenulatus 4-nitrophenol + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 40000, SDS-PAGE Geobacillus thermocatenulatus
3.1.1.3 More different chemical classes of additives are tested to manipulate the aggregation behaviour of BTL2 lipase with the aim of obtaining a monodisperse sample to use for crystallization. For the process of concentration of BTL2 lipase in monomeric form, 2-propanol and dodecyl dimethylamine-N-oxide are necessary Geobacillus thermocatenulatus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 BTL2 lipase
-
 Geobacillus thermocatenulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 60
-
 assay at Geobacillus thermocatenulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 7.5
-
 assay at Geobacillus thermocatenulatus