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Literature summary extracted from

  • Soares da Costa, T.P.; Yap, M.Y.; Perugini, M.A.; Wallace, J.C.; Abell, A.D.; Wilce, M.C.; Polyak, S.W.; Booker, G.W.
    Dual roles of F123 in protein homodimerization and inhibitor binding to biotin protein ligase from Staphylococcus aureus (2014), Mol. Microbiol., 91, 110-120.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.15 F123G the mutation has minimal effect upon the Km value for MgATP and biotin. In contrast, the kcat for biotin and MgATP are significantly compromised to about 10% that of wild type enzyme Staphylococcus aureus
6.3.4.15 F123R the mutation has minimal effect upon the Km value for MgATP and biotin. In contrast, the kcat for biotin and MgATP are significantly compromised to about 10% that of wild type enzyme Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.15 biotin acetylene i.e. (3aS,4S,6aR)-4-hept-6-ynyl-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-2-one Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.15 Mg2+ required for activity Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.15 38300
-
 2 * 38300, calculated from amino acid sequence Staphylococcus aureus
6.3.4.15 76000
-
 sedimentation equilibrium analysis Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Staphylococcus aureus
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] Staphylococcus aureus ECT-R2
-
 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Staphylococcus aureus
-
-
-
6.3.4.15 Staphylococcus aureus ECT-R2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15 Ni-NTA column chromatography, and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Staphylococcus aureus AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Staphylococcus aureus ECT-R2 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.15 homodimer 2 * 38300, calculated from amino acid sequence Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin protein ligase
-
 Staphylococcus aureus
6.3.4.15 BPL
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.4.15 0.0003
-
 biotin acetylene wild type enzyme, pH and temperature not specified in the publication Staphylococcus aureus
6.3.4.15 0.0072
-
 biotin acetylene mutant enzyme F123G, pH and temperature not specified in the publication Staphylococcus aureus
6.3.4.15 0.0076
-
 biotin acetylene mutant enzyme F123R, pH and temperature not specified in the publication Staphylococcus aureus