EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.375 | oxaloacetate | above 0.3 mM | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.375 | 0.02 | - |
NADPH | 70°C, pH 8.0 | Methanocaldococcus jannaschii | |
1.1.1.375 | 0.06 | - |
oxaloacetate | 70°C, pH 8.0 | Methanocaldococcus jannaschii | |
1.1.1.375 | 0.14 | - |
NADH | 70°C, pH 8.0 | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.375 | Ca2+ | in the presence of NADPH, 10 mM MgCl2, MnCl2 or CaCl2 is required to support full activity. When using NADH as coenzyme enzymatic activity is insensitive to salt concentration | Methanocaldococcus jannaschii | |
1.1.1.375 | K+ | in the presence of NADPH, full enzymatic activity requires a minimum salt concentration of 0.1 M NaCl or KCl. At lower salt concentrations, the activity decreases by a factor of three. When using NADH as coenzyme enzymatic activity is insensitive to salt concentration | Methanocaldococcus jannaschii | |
1.1.1.375 | Mg2+ | in the presence of NADPH, 10 mM MgCl2, MnCl2 or CaCl2 is required to support full activity. When using NADH as coenzyme enzymatic activity is insensitive to salt concentration | Methanocaldococcus jannaschii | |
1.1.1.375 | Mn2+ | in the presence of NADPH, 10 mM MgCl2, MnCl2 or CaCl2 is required to support full activity. When using NADH as coenzyme enzymatic activity is insensitive to salt concentration | Methanocaldococcus jannaschii | |
1.1.1.375 | Na+ | in the presence of NADPH, full enzymatic activity requires a minimum salt concentration of 0.1 M NaCl or KCl. At lower salt concentrations, the activity decreases by a factor of three. When using NADH as coenzyme enzymatic activity is insensitive to salt concentration | Methanocaldococcus jannaschii |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.1.1.375 | guanidine-HCl | transition between folded and unfolded enzyme (monitored by the change in molar ellipticity at 222 nm) is found at 2 M guanidine hydrochloride | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.375 | Methanocaldococcus jannaschii | Q60176 | - |
- |
1.1.1.375 | Methanocaldococcus jannaschii DSM 2661 | Q60176 | - |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.1.1.375 | the enzyme is not sensitive to oxygen | Methanocaldococcus jannaschii |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.375 | - |
Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.375 | oxaloacetate + NADH + H+ | - |
Methanocaldococcus jannaschii | (S)-malate + NAD+ | - |
r | |
1.1.1.375 | oxaloacetate + NADH + H+ | - |
Methanocaldococcus jannaschii DSM 2661 | (S)-malate + NAD+ | - |
r | |
1.1.1.375 | oxaloacetate + NADPH + H+ | - |
Methanocaldococcus jannaschii | (S)-malate + NADP+ | - |
r | |
1.1.1.375 | oxaloacetate + NADPH + H+ | - |
Methanocaldococcus jannaschii DSM 2661 | (S)-malate + NADP+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.375 | MJ0409 | locus name | Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.375 | 70 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.375 | 80 | - |
thermal transition between active and inactive enzyme starts at about 80°C and follows first-order kinetics | Methanocaldococcus jannaschii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.375 | NADH | NADPH is the preferred coenzyme | Methanocaldococcus jannaschii | |
1.1.1.375 | NADPH | NADPH is the preferred coenzyme | Methanocaldococcus jannaschii |