Literature summary extracted from
Du, Q.; Cui, W.; Zhang, C.; Yu, D.
GmRFP1 encodes a previously unknown RING-type E3 ubiquitin ligase in soybean (Glycine max) (2010), Mol. Biol. Rep., 37, 685-693.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.2.27 |
- |
Glycine max |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.3.2.27 |
C194S |
mutation in highly conserved residue, loss of ubiqitin ligase activity |
Glycine max |
2.3.2.27 |
C197S |
mutation in highly conserved residue, loss of ubiqitin ligase activity |
Glycine max |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.3.2.27 |
membrane |
the protein contains a RING-H2 motif and an N-terminal transmembrane domain |
Glycine max |
16020 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.3.2.27 |
43118 |
- |
x * 43118, calculated |
Glycine max |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.2.27 |
Glycine max |
C6EUD3 |
isoform RFP1 |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.3.2.27 |
additional information |
transcript is detected in all organs analyzed |
Glycine max |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.2.27 |
[RFP1-ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [RFP1]-L-lysine |
in the presence of ubiquitin, ATP, rabbit E1, human E2 and recombinant isoform RFP1, accumulation of high molecular weight ubiquitinated RFP1 is detected, indicating its ability to autoubiquitinate |
Glycine max |
[RFP1-ubiquitin-carrier protein E2]-L-cysteine + [RFP1]-N6-ubiquitinyl-L-lysine |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.3.2.27 |
? |
x * 43118, calculated |
Glycine max |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.2.27 |
RFP1 |
- |
Glycine max |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
2.3.2.27 |
Glycine max |
calculated |
- |
6.8 |
Expression
EC Number |
Organism |
Comment |
Expression |
---|
2.3.2.27 |
Glycine max |
trancription is downregulated by cold and drought treatments |
down |
2.3.2.27 |
Glycine max |
trancription is upregulated by abscisic acid and salt stress |
up |