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Literature summary extracted from

  • Du, Q.; Cui, W.; Zhang, C.; Yu, D.
    GmRFP1 encodes a previously unknown RING-type E3 ubiquitin ligase in soybean (Glycine max) (2010), Mol. Biol. Rep., 37, 685-693.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.27
-
Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.27 C194S mutation in highly conserved residue, loss of ubiqitin ligase activity Glycine max
2.3.2.27 C197S mutation in highly conserved residue, loss of ubiqitin ligase activity Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.27 membrane the protein contains a RING-H2 motif and an N-terminal transmembrane domain Glycine max 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.27 43118
-
x * 43118, calculated Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.27 Glycine max C6EUD3 isoform RFP1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.27 additional information transcript is detected in all organs analyzed Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.27 [RFP1-ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [RFP1]-L-lysine in the presence of ubiquitin, ATP, rabbit E1, human E2 and recombinant isoform RFP1, accumulation of high molecular weight ubiquitinated RFP1 is detected, indicating its ability to autoubiquitinate Glycine max [RFP1-ubiquitin-carrier protein E2]-L-cysteine + [RFP1]-N6-ubiquitinyl-L-lysine
-
?

Subunits

EC Number Subunits Comment Organism
2.3.2.27 ? x * 43118, calculated Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.3.2.27 RFP1
-
Glycine max

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.2.27 Glycine max calculated
-
6.8

Expression

EC Number Organism Comment Expression
2.3.2.27 Glycine max trancription is downregulated by cold and drought treatments down
2.3.2.27 Glycine max trancription is upregulated by abscisic acid and salt stress up