EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.6 | gene cadA, the cadA gene of is located in a gene cluster together with two transporters, one a putative mitochondrial carrier, the other a putative plasma membrane carrier, cloning in Escherichia coli strain TOP10, heterologous mitochondrial and cytosolic expression of the enzyme in Aspergillus niger strain ATCC 1015, the mitochondrial expression doubles the enzyme productivity compared to the cytosolic expression. Co-expression of a cytosolic or a mitochondrial aconitase with cytosolic CadA leads to a significant increase of itaconic acid production compared to cytsolic CadA expression alone. The rate-limiting step for itaconic acid production under these conditions appears to be both the provision of cis-aconitate as a substrate for the CadA as well as the CAD activity itself | Aspergillus terreus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.1.6 | cytosol | cytosolic isozyme | Aspergillus terreus | 5829 | - |
4.1.1.6 | mitochondrion | mitochondrial isozyme | Aspergillus terreus | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.6 | cis-aconitate | Aspergillus terreus | - |
itaconate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.6 | Aspergillus terreus | - |
gene cadA | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.6 | cis-aconitate | - |
Aspergillus terreus | itaconate + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.6 | CAD | - |
Aspergillus terreus |
4.1.1.6 | cis-aconitate decarboxylase | - |
Aspergillus terreus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.6 | 37 | - |
assay at | Aspergillus terreus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.6 | 80 | - |
recombinant enzyme in cell free extract, 20 min, inactivation | Aspergillus terreus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.6 | 6.2 | - |
assay at | Aspergillus terreus |