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Literature summary extracted from

  • Labonte, J.W.; Kudo, F.; Freeman, M.F.; Raber, M.L.; Townsend, C.A.
    Engineering the synthetic potential of beta-lactam synthetase and the importance of catalytic loop dynamics (2012), MedChemComm, 3, 960-966.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.3.3.4 H447A the mutant shows severely reduced turnover number compared to the wild type enzyme Streptomyces clavuligerus
6.3.3.4 V446A the mutant shows reduced turnover number compared to the wild type enzyme Streptomyces clavuligerus
6.3.3.4 V446G the mutant shows strongly reduced turnover number compared to the wild type enzyme Streptomyces clavuligerus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.4 0.037
-
 L-N2-(2-carboxyethyl)arginine wild type enzyme, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.04
-
 (2'R)-methyl-L-N2-(2-carboxyethyl)arginine wild type enzyme, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.04
-
 (2'S)-ethyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.06
-
 (2'S)-methyl-L-N2-(2-carboxyethyl)arginine wild type enzyme, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.06
-
 (2'S)-ethyl-L-N2-(2-carboxyethyl)arginine wild type enzyme, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.08
-
 (2'S)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme H447A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.33
-
 (2'R)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.36
-
 L-N2-(2-carboxyethyl)arginine mutant enzyme V446A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.46
-
 (2'S)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.6 1 L-N2-(2-carboxyethyl)arginine mutant enzyme V446G, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.9
-
 L-N2-(2-carboxyethyl)arginine mutant enzyme H447A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 1.2
-
 (2'S)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446G, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 1.9
-
 (2'R)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446G, pH and temperature not specified in the publication Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.3.4 ATP + L-N2-(2-carboxyethyl)arginine Streptomyces clavuligerus
-
 AMP + diphosphate + deoxyamidinoproclavaminate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.4 Streptomyces clavuligerus P0DJQ7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.4 ATP + (2'R)-methyl-L-N2-(2-carboxyethyl)arginine
-
 Streptomyces clavuligerus ?
-
 ?
6.3.3.4 ATP + (2'S)-ethyl-L-N2-(2-carboxyethyl)arginine
-
 Streptomyces clavuligerus ?
-
 ?
6.3.3.4 ATP + (2'S)-methyl-L-N2-(2-carboxyethyl)arginine
-
 Streptomyces clavuligerus ?
-
 ?
6.3.3.4 ATP + L-N2-(2-carboxyethyl)arginine
-
 Streptomyces clavuligerus AMP + diphosphate + deoxyamidinoproclavaminate
-
 ?
6.3.3.4 additional information no activity with (2'R)-ethyl-L-N2-(2-carboxyethyl)arginine Streptomyces clavuligerus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.3.4 beta-lactam-synthetase
-
 Streptomyces clavuligerus
6.3.3.4 betaLS
-
 Streptomyces clavuligerus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.3.4 0.0023
-
 (2'S)-ethyl-L-N2-(2-carboxyethyl)arginine wild type enzyme, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.004
-
 (2'S)-ethyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.013
-
 (2'S)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme H447A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.014
-
 (2'R)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.016
-
 L-N2-(2-carboxyethyl)arginine mutant enzyme H447A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.023
-
 (2'S)-methyl-L-N2-(2-carboxyethyl)arginine wild type enzyme, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.032
-
 (2'R)-methyl-L-N2-(2-carboxyethyl)arginine wild type enzyme, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.07
-
 (2'R)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446G, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.13
-
 L-N2-(2-carboxyethyl)arginine mutant enzyme V446G, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.13
-
 (2'S)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446G, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.28
-
 L-N2-(2-carboxyethyl)arginine mutant enzyme V446A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.34
-
 (2'S)-methyl-L-N2-(2-carboxyethyl)arginine mutant enzyme V446A, pH and temperature not specified in the publication Streptomyces clavuligerus
6.3.3.4 0.87
-
 L-N2-(2-carboxyethyl)arginine wild type enzyme, pH and temperature not specified in the publication Streptomyces clavuligerus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.3.4 ATP
-
 Streptomyces clavuligerus