Literature summary extracted from
de la Paz Santangelo, M.; Gest, P.M.; Guerin, M.E.; Coincon, M.; Pham, H.; Ryan, G.; Puckett, S.E.; Spencer, J.S.; Gonzalez-Juarrero, M.; Daher, R.; Lenaerts, A.J.; Schnappinger, D.; Therisod, M.; Ehrt, S.; Sygusch, J.; Jackson, M.
Glycolytic and non-glycolytic functions of Mycobacterium tuberculosis fructose-1,6-bisphosphate aldolase, an essential enzyme produced by replicating and non-replicating bacilli (2011), J. Biol. Chem., 286, 40219-40231.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.1.2.13 |
native form and in complex with a hydroxamate substrate analog, to 2.35- and 1.9 A resolution, respectively. Inhibitor attachment has no effect on the plasminogen binding activity of the enzyme, it competes with the natural substrate, fructose 1,6-bisphosphate, and substantiates a reaction mechanism associated with metallodependent aldolases involving recruitment of the catalytic zinc ion by the substrate upon active site binding |
Mycobacterium tuberculosis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.1.2.13 |
N-(4-hydroxybutyl)-glycolohydroxamic acid bisphosphate |
inhibitor attachment has no effect on the plasminogen binding activity of the enzyme but competes with the natural substrate, fructose 1,6-bisphosphate, and substantiates a reaction mechanism associated with metallodependent aldolases involving recruitment of the catalytic zinc ion by the substrate upon active site binding |
Mycobacterium tuberculosis |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
4.1.2.13 |
cell surface |
- |
Mycobacterium tuberculosis |
9986 |
- |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.2.13 |
Mycobacterium tuberculosis |
P9WQA3 |
- |
- |
Expression
EC Number |
Organism |
Comment |
Expression |
---|
4.1.2.13 |
Mycobacterium tuberculosis |
protein is produced under various axenic growth conditions including oxygen depletion and hence by non-replicating bacilli, and also in vivo in the lungs of infected guinea pigs and mice. Enzyme binds human plasmin(ogen) and protects bound plasmin from regulation by alpha2-antiplasmin |
additional information |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
4.1.2.13 |
physiological function |
gene product is required for the growth of Mycobacterium tuberculosis on gluconeogenetic substrates and in glucose-containing medium |
Mycobacterium tuberculosis |