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Literature summary extracted from

  • ChapetonMontes, D.; Candela, T.; Collignon, A.; Janoir, C.
    Localization of the Clostridium difficile cysteine protease Cwp84 and insights into its maturation process (2011), J. Bacteriol., 193, 5314-5321.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.15 additional information expression of the mature protein, amino acids 92-518, does not give an active protein. A protein fragment, amino acids 92-518, does not display proteolytic activity. A fragment including the propeptide, amino acids 30-518, displays proteolytic activity on azocasein and fibronectin and can be processed to the active mature protein Clostridioides difficile

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.15 cell surface main localization. The 80-kDa precursor protein is noncovalently associated with the S-layer proteins, while the 47-kDa mature form is tightly associated with the underlying cell wall Clostridioides difficile 9986
-
3.4.22.15 extracellular minor localization Clostridioides difficile
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 47000
-
 x * 80000, precursor, x * 47000, mature form, SDS-PAGE Clostridioides difficile
3.4.22.15 80000
-
 x * 80000, precursor, x * 47000, mature form, SDS-PAGE Clostridioides difficile

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Clostridioides difficile Q83UF3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.15 proteolytic modification the maturation of the recombinant protease Cwp8430-803 is a sequential process beginning at the C-terminal end, followed by one or more cleavages at the N-terminal end. The processing sites of recombinant Cwp84 are likely to be residues S92 and K518 Clostridioides difficile

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 azocasein + H2O
-
 Clostridioides difficile ?
-
 ?
3.4.22.15 Fibronectin + H2O
-
 Clostridioides difficile ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 ? x * 80000, precursor, x * 47000, mature form, SDS-PAGE Clostridioides difficile

Synonyms

EC Number Synonyms Comment Organism
3.4.22.15 Cwp84
-
 Clostridioides difficile