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Literature summary extracted from

  • Hajnal, I.; Lyskowski, A.; Hanefeld, U.; Gruber, K.; Schwab, H.; Steiner, K.
    Biochemical and structural characterization of a novel bacterial manganese-dependent hydroxynitrile lyase (2013), FEBS J., 280, 5815-5828.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.10
-
 Granulicella tundricola

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.10 to 2.5 A resolution, Structure exhibits a cupin fold, and manganese is bound to three histidine and one glutamine residue Granulicella tundricola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 10.3
-
 (R)-mandelonitrile pH 4.5, 25°C Granulicella tundricola

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.10 Mn2+ required Granulicella tundricola

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Granulicella tundricola E8WYN5
-
-
4.1.2.10 Granulicella tundricola MP5ACTX9 E8WYN5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.10 1.74
-
 pH 6.0, 25°C Granulicella tundricola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Granulicella tundricola cyanide + benzaldehyde
-
 r
4.1.2.10 (R)-mandelonitrile
-
 Granulicella tundricola MP5ACTX9 cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + benzaldehyde
-
 Granulicella tundricola (R)-mandelonitrile 90% enantiomeric excess at 80% conversion using 0.5 M benzaldehyde in a biphasic reaction system with methyl tertiary butyl ether r
4.1.2.10 cyanide + benzaldehyde
-
 Granulicella tundricola MP5ACTX9 (R)-mandelonitrile 90% enantiomeric excess at 80% conversion using 0.5 M benzaldehyde in a biphasic reaction system with methyl tertiary butyl ether r

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 AciX9_0562 locus name Granulicella tundricola
4.1.2.10 cupin 2 conserved barrel domain protein
-
 Granulicella tundricola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.10 0.03
-
 (R)-mandelonitrile pH 4.5, 25°C Granulicella tundricola