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Literature summary extracted from

  • Römisch-Margl, W.; Eisenreich, W.; Haase, I.; Bacher, A.; Fischer, M.
    2,5-Diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthases of fungi and archaea (2008), FEBS J., 275, 4403-4414.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.302 expression in Escherichia coli Aquifex aeolicus
1.1.1.302 expression in Escherichia coli [Candida] glabrata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.302 25300
-
 2 * 25300, SDS-PAGE Aquifex aeolicus
1.1.1.302 28500
-
 2 * 28500, SDS-PAGE [Candida] glabrata
1.1.1.302 50600
-
 sedimentation equilibrium analysis Aquifex aeolicus
1.1.1.302 57800
-
 sedimentation equilibrium analysis [Candida] glabrata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ Methanocaldococcus jannaschii biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ Aquifex aeolicus biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ [Candida] glabrata biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ Methanocaldococcus jannaschii DSM 2661 biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ [Candida] glabrata ATCC 2001 biosynthesis of riboflavin 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.302 Aquifex aeolicus O66747
-
-
1.1.1.302 Methanocaldococcus jannaschii Q58085
-
-
1.1.1.302 Methanocaldococcus jannaschii DSM 2661 Q58085
-
-
1.1.1.302 [Candida] glabrata Q6FU96
-
-
1.1.1.302 [Candida] glabrata ATCC 2001 Q6FU96
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.302
-
 Aquifex aeolicus
1.1.1.302
-
 [Candida] glabrata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin Methanocaldococcus jannaschii 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin Aquifex aeolicus 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin [Candida] glabrata 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation Aquifex aeolicus 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate Methanocaldococcus jannaschii 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate [Candida] glabrata 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin Methanocaldococcus jannaschii DSM 2661 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate Methanocaldococcus jannaschii DSM 2661 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ biosynthesis of riboflavin [Candida] glabrata ATCC 2001 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?
1.1.1.302 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NADPH + H+ the beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerisation. The enzyme is an A-type reductase catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate [Candida] glabrata ATCC 2001 2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.302 homodimer 2 * 28500, SDS-PAGE [Candida] glabrata
1.1.1.302 homodimer 2 * 25300, SDS-PAGE Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.302 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase
-
 Methanocaldococcus jannaschii
1.1.1.302 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase
-
 Aquifex aeolicus
1.1.1.302 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase
-
 [Candida] glabrata
1.1.1.302 arfC
-
 Methanocaldococcus jannaschii
1.1.1.302 MJ0671 locus name Methanocaldococcus jannaschii
1.1.1.302 ribD2
-
 Aquifex aeolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.302 37
-
 assay at Methanocaldococcus jannaschii
1.1.1.302 37
-
 assay at Aquifex aeolicus
1.1.1.302 37
-
 assay at [Candida] glabrata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.302 8.2
-
 assay at Methanocaldococcus jannaschii
1.1.1.302 8.2
-
 assay at [Candida] glabrata
1.1.1.302 8.2
-
 asay at Aquifex aeolicus

General Information

EC Number General Information Comment Organism
1.1.1.302 physiological function biosynthesis of riboflavin Methanocaldococcus jannaschii
1.1.1.302 physiological function biosynthesis of riboflavin Aquifex aeolicus
1.1.1.302 physiological function biosynthesis of riboflavin [Candida] glabrata