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Literature summary extracted from

  • Pegan, S.D.; Rukseree, K.; Capodagli, G.C.; Baker, E.A.; Krasnykh, O.; Franzblau, S.G.; Mesecar, A.D.
    Active site loop dynamics of a class IIa fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis (2013), Biochemistry, 52, 912-925.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.13 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.13 in complex with inhibitor phosphoglycolohydroxamate as a mimic of the hydroxyenolate intermediate- and dihydroxyacetone phosphate-bound form of the enzyme, to 1.58 A resolution. Residue E169 facilitates a water-mediated deprotonation-protonation step of the reaction mechanism Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.13 D276A about 1% of wild-type activity Mycobacterium tuberculosis
4.1.2.13 E168A about 30% of wild-type activity Mycobacterium tuberculosis
4.1.2.13 E169A almost complete loss of activity Mycobacterium tuberculosis
4.1.2.13 G167A about 3% of wild-type activity Mycobacterium tuberculosis
4.1.2.13 G167A/G166A about 2% of wild-type activity Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.13 dihydroxyacetone phosphate competitive with fructose 1,6-bisphosphate Mycobacterium tuberculosis
4.1.2.13 Phosphoglycolohydroxamate use as a mimic of the hydroxyenolate intermediate- and dihydroxyacetone phosphate-bound form of the enzyme Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.018
-
 D-fructose-1,6-bisphosphate mutant E169A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 0.021
-
 D-fructose-1,6-bisphosphate mutant G167A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 0.021
-
 D-fructose-1,6-bisphosphate mutant G167A/G166A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 0.03
-
 D-fructose-1,6-bisphosphate mutant E168A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 0.04
-
 D-fructose-1,6-bisphosphate wild-type, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 0.18
-
 D-fructose-1,6-bisphosphate mutant D276A, pH 7.8, 22°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Mycobacterium tuberculosis P9WQA3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose-1,6-bisphosphate
-
 Mycobacterium tuberculosis dihydroxyacetone phosphate + glyceraldehyde-3-phosphate
-
 ?
4.1.2.13 additional information product glycerone phosphate is leaving first followed by dihydroxyacetone phosphate in a uni-bi kinetic scheme Mycobacterium tuberculosis ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.13 0.01
-
 D-fructose-1,6-bisphosphate mutant E169A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 0.11
-
 D-fructose-1,6-bisphosphate mutant G167A/G166A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 0.17
-
 D-fructose-1,6-bisphosphate mutant G167A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 0.45
-
 D-fructose-1,6-bisphosphate mutant D276A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 2.67
-
 D-fructose-1,6-bisphosphate mutant E168A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 12
-
 D-fructose-1,6-bisphosphate wild-type, pH 7.8, 22°C Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.13 0.238
-
 dihydroxyacetone phosphate wild-type, pH 7.8, 22°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.13 0.28
-
 D-fructose-1,6-bisphosphate mutant E169A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 2.5
-
 D-fructose-1,6-bisphosphate mutant D276A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 5.33
-
 D-fructose-1,6-bisphosphate mutant G167A/G166A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 8
-
 D-fructose-1,6-bisphosphate mutant G167A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 88.33
-
 D-fructose-1,6-bisphosphate mutant E168A, pH 7.8, 22°C Mycobacterium tuberculosis
4.1.2.13 300
-
 D-fructose-1,6-bisphosphate wild-type, pH 7.8, 22°C Mycobacterium tuberculosis