Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Alves, J.; Westling, L.; Peters, E.C.; Harris, J.L.; Trauger, J.W.
    Cloning, expression, and enzymatic activity of Acinetobacter baumannii and Klebsiella pneumoniae acetyl-coenzyme A carboxylases (2011), Anal. Biochem., 417, 103-111.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.2 expressed in Escherichia coli as a His-tagged fusion protein Klebsiella pneumoniae
6.4.1.2 expressed in Escherichia coli as a His-tagged fusion protein Acinetobacter baumannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 pseudopeptide pyrrolidinedione inhibitor
-
 Acinetobacter baumannii
6.4.1.2 pseudopeptide pyrrolidinedione inhibitor
-
 Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.0088
-
 acetyl-CoA pH 8, 20°C Acinetobacter baumannii
6.4.1.2 0.0487
-
 acetyl-CoA pH 8, 20°C Klebsiella pneumoniae
6.4.1.2 0.083
-
 ATP pH 8, 20°C Klebsiella pneumoniae
6.4.1.2 0.1037
-
 ATP pH 8, 20°C Acinetobacter baumannii

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Acinetobacter baumannii
-
-
-
6.4.1.2 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2 using Ni-NTA chromatography Klebsiella pneumoniae
6.4.1.2 using Ni-NTA chromatography Acinetobacter baumannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Klebsiella pneumoniae ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Acinetobacter baumannii ADP + phosphate + malonyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.4.1.2 ACCase
-
 Klebsiella pneumoniae
6.4.1.2 ACCase
-
 Acinetobacter baumannii
6.4.1.2 Acetyl-coenzyme A carboxylase
-
 Klebsiella pneumoniae
6.4.1.2 Acetyl-coenzyme A carboxylase
-
 Acinetobacter baumannii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.4.1.2 11.6
-
 acetyl-CoA co-substrate: ATP, HCO3-, pH 8, 20°C Acinetobacter baumannii
6.4.1.2 30.1
-
 acetyl-CoA co-substrate: ATP, HCO3-, pH 8, 20°C Klebsiella pneumoniae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.4.1.2 0.000008
-
 pH 8, 20°C Klebsiella pneumoniae pseudopeptide pyrrolidinedione inhibitor
6.4.1.2 0.000033
-
 pH 8, 20°C Acinetobacter baumannii pseudopeptide pyrrolidinedione inhibitor