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Literature summary extracted from

  • Izzo, V.; Notomista, E.; Picardi, A.; Pennacchio, F.; di Donato, A.
    The thermophilic archaeon Sulfolobus solfataricus is able to grow on phenol (2005), Res. Microbiol., 156, 677-689.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0016
-
 catechol pH 7.5, 25°C Saccharolobus solfataricus
1.13.11.2 0.0017
-
 3-methylcatechol pH 7.5,25 °C Saccharolobus solfataricus
1.13.11.2 0.003
-
 4-methylcatechol pH 7.5, 25°C Saccharolobus solfataricus
1.13.11.2 0.0036
-
 3-methylcatechol pH 7.5, 60°C Saccharolobus solfataricus
1.13.11.2 0.0059
-
 catechol pH 7.5, 60°C Saccharolobus solfataricus
1.13.11.2 0.012
-
 4-methylcatechol pH 7.5, 60°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.2 Fe2+ the enzyme contains about 2 mol of Fe2+ per mole of protein Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Saccharolobus solfataricus the enzyme is constitutively transcribed 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2 Saccharolobus solfataricus P2 the enzyme is constitutively transcribed 2-hydroxymuconate-6-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Saccharolobus solfataricus V5W6K5
-
-
1.13.11.2 Saccharolobus solfataricus P2 V5W6K5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
 Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
1.13.11.2 4°C, the enzyme retains complete activity for more than 350 min when kept under an N2 atmosphere, whereas it loses about 80% of its activity when stored for the same time in the absence of nitrogen Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2
-
 Saccharolobus solfataricus ?
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Saccharolobus solfataricus P2 ?
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Saccharolobus solfataricus ?
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Saccharolobus solfataricus P2 ?
-
 ?
1.13.11.2 catechol + O2
-
 Saccharolobus solfataricus 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2 the enzyme is constitutively transcribed Saccharolobus solfataricus 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Saccharolobus solfataricus P2 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2 the enzyme is constitutively transcribed Saccharolobus solfataricus P2 2-hydroxymuconate-6-semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 SSO1223 locus name Saccharolobus solfataricus
1.13.11.2 SsoC2,3O
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.2 70 80
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.2 50 80 50°C: about 50% of maximal activity, 70-80°C: maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.2 60
-
 half-life: 230-240 min Saccharolobus solfataricus
1.13.11.2 70
-
 half-life: 100-110 min Saccharolobus solfataricus
1.13.11.2 80
-
 half-life: about 20 min Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 0.58
-
 3-methylcatechol pH 7.5,25 °C Saccharolobus solfataricus
1.13.11.2 0.76
-
 4-methylcatechol pH 7.5, 25°C Saccharolobus solfataricus
1.13.11.2 1.3
-
 catechol pH 7.5, 25°C Saccharolobus solfataricus
1.13.11.2 4.7
-
 4-methylcatechol pH 7.5, 60°C Saccharolobus solfataricus
1.13.11.2 5.2
-
 3-methylcatechol pH 7.5, 60°C Saccharolobus solfataricus
1.13.11.2 7.8
-
 catechol pH 7.5, 60°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 7.5
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.2 250
-
 4-methylcatechol pH 7.5, 25°C Saccharolobus solfataricus
1.13.11.2 340
-
 3-methylcatechol pH 7.5,25 °C Saccharolobus solfataricus
1.13.11.2 400
-
 4-methylcatechol pH 7.5, 60°C Saccharolobus solfataricus
1.13.11.2 810
-
 catechol pH 7.5, 25°C Saccharolobus solfataricus
1.13.11.2 1300
-
 catechol pH 7.5, 60°C Saccharolobus solfataricus
1.13.11.2 1400
-
 3-methylcatechol pH 7.5, 60°C Saccharolobus solfataricus