Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zheng, H.; Beliavsky, A.; Tchigvintsev, A.; Brunzelle, J.S.; Brown, G.; Flick, R.; Evdokimova, E.; Wawrzak, Z.; Mahadevan, R.; Anderson, W.F.; Savchenko, A.; Yakunin, A.F.
    Structure and activity of the NAD(P)+-dependent succinate semialdehyde dehydrogenase YneI from Salmonella typhimurium (2013), Proteins, 81, 1031-1041.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.16 dithiothreitol the enzyme is stimulated (up to 40%) by the addition of dithiothreitol (0.1-0.2 mM) Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.16 expressed in Escherichia coli BL21 cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.16 apo-form and in complex with NAD+, hanging drop vapor diffusion method, using 2 M sodium formate and 0.1 M sodium acetate (pH 4.6), at 22°C Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.16 D426A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 E442A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 F138A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 K160A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 K160E the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 K92A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 N193A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 R145A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 R428A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 S425A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 W141A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.16 succinate semialdehyde substrate inhibition at concentrations higher than 0.1 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 0.022
-
 succinate semialdehyde mutant enzyme R428A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.027
-
 succinate semialdehyde wild type enzyme, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.043
-
 NAD+ wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.047
-
 succinate semialdehyde wild type enzyme, with NADP+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.12
-
 succinate semialdehyde mutant enzyme F138A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.14
-
 succinate semialdehyde mutant enzyme E442A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.15
-
 NAD+ mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.22
-
 succinate semialdehyde mutant enzyme S425A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.23
-
 succinate semialdehyde mutant enzyme D426A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.482
-
 NADP+ wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.77
-
 succinate semialdehyde mutant enzyme W141A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.4
-
 NADP+ mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.49
-
 succinate semialdehyde mutant enzyme R145A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.6
-
 succinate semialdehyde mutant enzyme K92A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 10
-
 NAD+ mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 11
-
 NAD+ mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 26
-
 NADP+ mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 27
-
 NADP+ mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.16 49000
-
 2 * 49000 , predicted from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 84000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.16 2-naphthaldehyde + NAD+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium low dehydrogenase activity 2-naphthoate + NADH + H+
-
 ?
1.2.1.16 2-naphthaldehyde + NADP+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium low dehydrogenase activity 2-naphthoate + NADPH + H+
-
 ?
1.2.1.16 decanal + NAD+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium low dehydrogenase activity decanoate + NADH + H+
-
 ?
1.2.1.16 decanal + NADP+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium low dehydrogenase activity decanoate + NADPH + H+
-
 ?
1.2.1.16 additional information Salmonella enterica subsp. enterica serovar Typhimurium the enzyme shows no detectable activity with hexanal or benzaldehyde ?
-
 ?
1.2.1.16 succinate semialdehyde + NAD+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 succinate + NADH + H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 succinate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZPI3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.16 Ni-NTA resin column chromatography and Superdex 75 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 2-naphthaldehyde + NAD+ + H2O low dehydrogenase activity Salmonella enterica subsp. enterica serovar Typhimurium 2-naphthoate + NADH + H+
-
 ?
1.2.1.16 2-naphthaldehyde + NADP+ + H2O low dehydrogenase activity Salmonella enterica subsp. enterica serovar Typhimurium 2-naphthoate + NADPH + H+
-
 ?
1.2.1.16 decanal + NAD+ + H2O low dehydrogenase activity Salmonella enterica subsp. enterica serovar Typhimurium decanoate + NADH + H+
-
 ?
1.2.1.16 decanal + NADP+ + H2O low dehydrogenase activity Salmonella enterica subsp. enterica serovar Typhimurium decanoate + NADPH + H+
-
 ?
1.2.1.16 additional information the enzyme shows no detectable activity with hexanal or benzaldehyde Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium succinate + NADH + H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium succinate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.16 homodimer 2 * 49000 , predicted from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
1.2.1.16 NAD(P)+-dependent succinate semialdehyde dehydrogenase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 SSADH
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 YneI
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.16 0.3
-
 succinate semialdehyde mutant enzyme D426A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2 8 succinate semialdehyde mutant enzyme R428A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.1
-
 NADP+ mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 2.6
-
 NADP+ mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 14
-
 NAD+ mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 21
-
 NADP+ mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 26
-
 succinate semialdehyde mutant enzyme F138A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 38
-
 NAD+ wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 39
-
 NAD+ mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 45
-
 succinate semialdehyde mutant enzyme R145A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 46
-
 NADP+ wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 55
-
 succinate semialdehyde wild type enzyme, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 66
-
 succinate semialdehyde mutant enzyme W141A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 68
-
 succinate semialdehyde mutant enzyme S425A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 73
-
 succinate semialdehyde mutant enzyme K92A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 80
-
 NAD+ mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 83
-
 succinate semialdehyde wild type enzyme, with NADP+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 164
-
 succinate semialdehyde mutant enzyme E442A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.16 7.5 9
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+ the enzyme can use both NAD+ and NADP+ although affinity to NAD+ is 10times higher Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 NADP+ the enzyme can use both NAD+ and NADP+ although affinity to NAD+ is 10times higher Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.16 0.077
-
 NADP+ mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 0.1
-
 NADP+ mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 3.8
-
 NAD+ mutant enzyme K160A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 7.3
-
 NAD+ mutant enzyme K160E, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 8.6
-
 NADP+ mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 18
-
 succinate semialdehyde mutant enzyme R145A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 28
-
 succinate semialdehyde mutant enzyme K92A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 86
-
 succinate semialdehyde mutant enzyme W141A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 95
-
 NADP+ wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 97
-
 NAD+ mutant enzyme N193A, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 110
-
 succinate semialdehyde mutant enzyme E442A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 130
-
 succinate semialdehyde mutant enzyme D426A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 130
-
 succinate semialdehyde mutant enzyme R428A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 180
-
 succinate semialdehyde wild type enzyme, with NADP+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 200
-
 succinate semialdehyde wild type enzyme, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 210
-
 succinate semialdehyde mutant enzyme F138A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 310
-
 succinate semialdehyde mutant enzyme S425A, with NAD+ as cosubstrate, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.16 880
-
 NAD+ wild type enzyme, in 50 mM HEPES (pH 8.0), at 37°C Salmonella enterica subsp. enterica serovar Typhimurium