EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.11 | expressed in Escherichia coli BL21(DE3) cells | Solanum lycopersicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.11 | C180S | the mutant of isoform E4 shows wild type activity | Solanum lycopersicum |
1.8.4.11 | C180S | the mutant of isoform MSRA2 shows strongly reduced catalytic efficiency compared to the wild type enzyme | Solanum lycopersicum |
1.8.4.11 | C188S | the mutant of isoform E4 shows strongly reduced catalytic efficiency compared to the wild type enzyme | Solanum lycopersicum |
1.8.4.11 | C194S | the mutant of isoform E4 shows strongly reduced catalytic efficiency compared to the wild type enzyme | Solanum lycopersicum |
1.8.4.11 | C37S | inactive | Solanum lycopersicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.11 | 0.00322 | - |
thioredoxin | wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.00343 | - |
thioredoxin | mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.0189 | - |
thioredoxin | mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.0218 | - |
thioredoxin | wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.02265 | - |
thioredoxin | mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.0233 | - |
thioredoxin | mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 22000 | - |
x * 22000, SDS-PAGE | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Solanum lycopersicum | - |
L-methionine + thioredoxin disulfide + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.11 | Solanum lycopersicum | G3JX11 | cultivar Micro-Tom | - |
1.8.4.11 | Solanum lycopersicum | P54153 | cultivar Micro-Tom | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.4.11 | fruit | - |
Solanum lycopersicum | - |
1.8.4.11 | leaf | - |
Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.11 | dabsyl-L-methionine (S)-sulfoxide + dithiothreitol | - |
Solanum lycopersicum | dabsyl-L-methionine + dithiothreitol disulfide + H2O | - |
? | |
1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | - |
Solanum lycopersicum | L-methionine + thioredoxin disulfide + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.4.11 | ? | x * 22000, SDS-PAGE | Solanum lycopersicum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.11 | methionine sulfoxide reductase A | - |
Solanum lycopersicum |
1.8.4.11 | MSRA2 | - |
Solanum lycopersicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.11 | 0.1 | - |
thioredoxin | mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.1 | - |
thioredoxin | mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.11 | - |
thioredoxin | wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.13 | - |
thioredoxin | mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.15 | - |
thioredoxin | wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 0.17 | - |
thioredoxin | mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.11 | thioredoxin | - |
Solanum lycopersicum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.8.4.11 | Solanum lycopersicum | the transcript level of isoform MSRA2 is significantly accumulated in both leaves and green fruits after ethephon treatment | up |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.11 | 4.3 | - |
thioredoxin | mutant C194S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 4.9 | - |
thioredoxin | mutant C180S of isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 5.1 | - |
thioredoxin | wild type isoform MSRA2, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 6.9 | - |
thioredoxin | mutant C188S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 46.5 | - |
thioredoxin | wild type isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum | |
1.8.4.11 | 49.5 | - |
thioredoxin | mutant C180S of isoform E4, in 50 mM Tris-HCl (pH 8.0), at 28°C | Solanum lycopersicum |