EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.244 | activator protein ACT | all three isozymes are activated by ACT, which is encoded by gene act, overview | Bacillus methanolicus | |
1.1.1.244 | NudF protein | can be catalytically stimulated by the Bacillus subtilis NudF protein in vitro | Bacillus methanolicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.244 | three isozymes, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain ER2566 | Bacillus methanolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.244 | 1 | - |
formaldehyde | pH 6.7, 45°C, isozyme Mdh3 | Bacillus methanolicus | |
1.1.1.244 | 1.1 | - |
formaldehyde | pH 6.7, 45°C, isozyme Mdh1 | Bacillus methanolicus | |
1.1.1.244 | 3 | - |
formaldehyde | pH 6.7, 45°C, isozyme Mdh1 | Bacillus methanolicus | |
1.1.1.244 | 4.5 | - |
formaldehyde | pH 6.7, 45°C, isozyme Mdh2 | Bacillus methanolicus | |
1.1.1.244 | 7 | - |
formaldehyde | pH 6.7, 45°C, isozyme Mdh2 | Bacillus methanolicus | |
1.1.1.244 | 7.1 | - |
formaldehyde | pH 6.7, 45°C, isozyme Mdh3 | Bacillus methanolicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.244 | methanol + NAD+ | Bacillus methanolicus | - |
formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | Bacillus methanolicus PB1 | - |
formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | Bacillus methanolicus MGA3 | - |
formaldehyde + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.244 | Bacillus methanolicus | - |
ATCC53907, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid | - |
1.1.1.244 | Bacillus methanolicus | - |
NCIMB13113, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid | - |
1.1.1.244 | Bacillus methanolicus MGA3 | - |
ATCC53907, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid | - |
1.1.1.244 | Bacillus methanolicus PB1 | - |
NCIMB13113, three isozymes, two of the genes are encoded on the chromosome and one on a plasmid | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.244 | 1,3-propandiol + NAD+ | very low activity | Bacillus methanolicus | ? + NADH + H+ | - |
? | |
1.1.1.244 | 1,3-propandiol + NAD+ | low activity | Bacillus methanolicus | ? + NADH + H+ | - |
? | |
1.1.1.244 | butanol + NAD+ | - |
Bacillus methanolicus | butanal + NADH + H+ | - |
? | |
1.1.1.244 | ethanol + NAD+ | - |
Bacillus methanolicus | acetaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | formaldehyde + NADH + H+ | Mdhs generally show higher activity and affinity for formaldehyde compared to methanol | Bacillus methanolicus | methanol + NAD+ | - |
r | |
1.1.1.244 | formaldehyde + NADH + H+ | Mdhs generally show higher activity and affinity for formaldehyde compared to methanol | Bacillus methanolicus PB1 | methanol + NAD+ | - |
r | |
1.1.1.244 | formaldehyde + NADH + H+ | Mdhs generally show higher activity and affinity for formaldehyde compared to methanol | Bacillus methanolicus MGA3 | methanol + NAD+ | - |
r | |
1.1.1.244 | isopropanol + NAD+ | low activity | Bacillus methanolicus | isopropanal + NADH + H+ | - |
? | |
1.1.1.244 | methanol + NAD+ | - |
Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | - |
Bacillus methanolicus PB1 | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol | Bacillus methanolicus PB1 | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol | Bacillus methanolicus PB1 | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | - |
Bacillus methanolicus MGA3 | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol | Bacillus methanolicus MGA3 | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | methanol + NAD+ | very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol | Bacillus methanolicus MGA3 | formaldehyde + NADH + H+ | - |
r | |
1.1.1.244 | additional information | all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes | Bacillus methanolicus | ? | - |
? | |
1.1.1.244 | additional information | all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes | Bacillus methanolicus PB1 | ? | - |
? | |
1.1.1.244 | additional information | all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes | Bacillus methanolicus MGA3 | ? | - |
? | |
1.1.1.244 | propanol + NAD+ | - |
Bacillus methanolicus | propanal + NADH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.244 | MDH | - |
Bacillus methanolicus |
1.1.1.244 | NAD+ dependent methanol dehydrogenase | - |
Bacillus methanolicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.244 | 45 | - |
activity optimum at about 45°C for all isozymes | Bacillus methanolicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.244 | 45 | - |
20 min, the isozymes are all stable up to | Bacillus methanolicus |
1.1.1.244 | 45 | - |
20 min, the isozymes are stable up to, except for isozyme Mdh1, which shows about 35-40% loss of activity | Bacillus methanolicus |
1.1.1.244 | 60 | - |
20 min, isozyme Mdh1 is inactivated, isozyme Mdh2 loses about 80% of activity, while isozyme Mdh3 retains over 80% of its activity | Bacillus methanolicus |
1.1.1.244 | 60 | - |
20 min, isozyme Mdh1 loses 90% activity, isozyme Mdh2 loses 80% of activity, while isozyme Mdh3 retains 60% of its activity | Bacillus methanolicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.244 | 6.7 | - |
formaldehyde reduction assay at | Bacillus methanolicus |
1.1.1.244 | 9.5 | - |
alcohol oxidation reaction assay at | Bacillus methanolicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.244 | NAD+ | dependent on | Bacillus methanolicus | |
1.1.1.244 | NADH | - |
Bacillus methanolicus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.244 | evolution | strain MGA3 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes | Bacillus methanolicus |
1.1.1.244 | evolution | strain PB1 contains three isozymes that belong to the type III Fe-NAD+-dependent alcohol dehydrogenases, but show a distinct substrate specificity and major differences with respect to transcriptional regulation of the paralogous genes | Bacillus methanolicus |
1.1.1.244 | physiological function | biological significance of Mdh for methanol oxidation during methylotrophic growth and biological role of the enzyme as part of a formaldehyde detoxification system in the methanol consuming cells | Bacillus methanolicus |