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Literature summary extracted from

  • van Lis, R.; Baffert, C.; Coute, Y.; Nitschke, W.; Atteia, A.
    Chlamydomonas reinhardtii chloroplasts contain a homodimeric pyruvate:ferredoxin oxidoreductase that functions with FDX1 (2013), Plant Physiol., 161, 57-71.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.7.1 additional information dithiothreitol has no influence on the activity Chlamydomonas reinhardtii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.7.1 expressed in Escherichia coli BL21(DE3) cells Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.7.1 NaCl the addition of 0.3 M NaCl causes a significant inhibition (up to 80%) of the activity Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.0037
-
 oxidized ferredoxin 2 in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
1.2.7.1 0.0046
-
 oxidized ferredoxin 1 in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
1.2.7.1 0.0052
-
 CoA in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
1.2.7.1 0.16
-
 pyruvate in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.7.1 chloroplast
-
 Chlamydomonas reinhardtii 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.1 Mg2+ upon addition of Mg2+, an ion that stabilizes thiamine diphosphate, the enzymatic activity almost doubles Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 130000
-
 2 * 130000, SDS-PAGE Chlamydomonas reinhardtii
1.2.7.1 235000
-
 recombinant enzyme, native PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.7.1 pyruvate + CoA + oxidized ferredoxin 1 Chlamydomonas reinhardtii
-
 acetyl-CoA + CO2 + reduced ferredoxin 1 + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized ferredoxin 1 Chlamydomonas reinhardtii CC124
-
 acetyl-CoA + CO2 + reduced ferredoxin 1 + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized ferredoxin 2 Chlamydomonas reinhardtii
-
 acetyl-CoA + CO2 + reduced ferredoxin 2 + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized ferredoxin 2 Chlamydomonas reinhardtii CC124
-
 acetyl-CoA + CO2 + reduced ferredoxin 2 + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Chlamydomonas reinhardtii
-
-
-
1.2.7.1 Chlamydomonas reinhardtii CC124
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.1 when the recombinant enzyme (in 50 mM Tricine, pH 8.0) is exposed to air for 1 h on ice, a drastic loss of activity is observed Chlamydomonas reinhardtii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1 nickel affinity column chromatography Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.1 0.4
-
 using cytochrome c as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
1.2.7.1 2.3
-
 using FMN as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
1.2.7.1 3.7
-
 using methyl viologen as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
1.2.7.1 4.5
-
 using spinach ferredoxin 1 as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
1.2.7.1 4.7
-
 using methylene blue as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
1.2.7.1 6.3
-
 using Chlamydomonas reinhardtii ferredoxin 1 as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii
1.2.7.1 12
-
 using benzyl viologen as electron acceptor, in 25 mM potassium phosphate buffer (pH 7.0), 0.2 mM thiamine diphosphate, 1 mM MgCl2, at 25 °C Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 additional information neither NAD+ nor NADP+ can be reduced by the enzyme Chlamydomonas reinhardtii ?
-
 ?
1.2.7.1 additional information neither NAD+ nor NADP+ can be reduced by the enzyme Chlamydomonas reinhardtii CC124 ?
-
 ?
1.2.7.1 pyruvate + CoA + FMN 5-6times lower activity with FMN as compared to benzyl viologen Chlamydomonas reinhardtii acetyl-CoA + CO2 + FMNH + H+
-
 ?
1.2.7.1 pyruvate + CoA + FMN 5-6times lower activity with FMN as compared to benzyl viologen Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + FMNH + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized benzyl viologen best activity with benzyl viologen Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced benzyl viologen + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized benzyl viologen best activity with benzyl viologen Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + reduced benzyl viologen + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized ferredoxin 1
-
 Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced ferredoxin 1 + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized ferredoxin 1
-
 Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + reduced ferredoxin 1 + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized ferredoxin 2
-
 Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced ferredoxin 2 + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized ferredoxin 2
-
 Chlamydomonas reinhardtii CC124 acetyl-CoA + CO2 + reduced ferredoxin 2 + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methyl viologen 3-4times lower activity with methyl viologen as compared to benzyl viologen Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced methyl viologen + H+
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methylene blue 2.5-3times lower activity with methylene blue as compared to benzyl viologen Chlamydomonas reinhardtii acetyl-CoA + CO2 + reduced methylene blue + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 homodimer 2 * 130000, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 PFOR
-
 Chlamydomonas reinhardtii
1.2.7.1 PFR
-
 Chlamydomonas reinhardtii
1.2.7.1 pyruvate:ferredoxin oxidoreductase
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 44
-
-
 Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.1 7 7.5 recombinant enzyme, in 25 mM potassium phosphate Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.1 thiamine diphosphate essential cofactor, upon addition of Mg2+, an ion that stabilizes thiamine diphosphate, the enzymatic activity almost doubles Chlamydomonas reinhardtii