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Literature summary extracted from

  • Ryden, A.; Ruyter-Spira, C.; Litjens, R.; Takahashi, S.; Quax, W.; Osada, H.; Bouwmeester, H.; Kayser, O.
    Molecular cloning and characterization of a broad substrate terpenoid oxidoreductase from Artemisia annua (2010), Plant Cell Physiol., 51, 1219-1228.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.208 expression in Escherichia coli Artemisia annua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.208 0.0071
-
 (-)-menthone pH 7.0, 30°C Artemisia annua
1.1.1.208 0.305
-
 (+)-neomenthol pH 7.0, 30°C Artemisia annua

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.208 Artemisia annua D1MI43
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.208 (+)-neomenthol + NADP+ reaction proceeds via (-)-menthone Artemisia annua (-)-menthone + NADPH + H+
-
 r
1.1.1.208 (-)-menthone + NADPH + H+
-
 Artemisia annua (+)-neomenthol + NADP+ main product, plus some (-)-menthol r
1.1.1.208 additional information no substrates for reduction: carvone and isopiperitenone. No substrates in dehydrogenase reaction: (+)-borneol, (-)-borneol, (+)-menthol and (-)-neomenthol Artemisia annua ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.208 Red 1
-
 Artemisia annua

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.208 0.6
-
 (-)-menthone pH 7.0, 30°C Artemisia annua
1.1.1.208 0.91
-
 (+)-neomenthol pH 7.0, 30°C Artemisia annua

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.208 NADPH
-
 Artemisia annua

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.208 2.99
-
 (+)-neomenthol pH 7.0, 30°C Artemisia annua
1.1.1.208 83.6
-
 (-)-menthone pH 7.0, 30°C Artemisia annua