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Literature summary extracted from

  • Matsushima, Y.; Minami, H.; Hori, K.; Sato, F.
    Pathway engineering of benzylisoquinoline alkaloid biosynthesis in transgenic California poppy cells with ectopic expression of tetrahydroberberine oxidase from Coptis japonica (2012), Plant Biotechnol., 29, 473-481.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.8 DNA and amino acid sequence determination and analysis, functional ectopical expression of CjTHBO under the control of Cauliflower mosaic virus 35S promoterin Eschscholzia californica cells via transformation with Agrobacterium tumefaciens strain LBA4404. The transgenic cells produce coptisine and dehydrocheilanthifoline, which are expected to be generated by the reaction of CjTHBO from pathway intermediates, i.e. cheilanthifoline and stylopine, alkaloid profile analysis in transgenic California poppy cells, overview. Expression in Escherichia coli strain BL21 (DE3) Coptis japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.8 57748
-
 x * 57748, sequence calculation Coptis japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.8 (S)-tetrahydroberberine + 2 O2 Coptis japonica
-
 berberine + 2 H2O2
-
 ?
1.3.3.8 additional information Coptis japonica the recombinant enzyme expressed in transgenic Eschscholzia californica cells produces coptisine and dehydrocheilanthifoline from pathway intermediates cheilanthifoline and stylopine, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.8 Coptis japonica E3WDH1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.3.8 cell culture
-
 Coptis japonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.8 (S)-tetrahydroberberine + 2 O2
-
 Coptis japonica berberine + 2 H2O2
-
 ?
1.3.3.8 additional information the recombinant enzyme expressed in transgenic Eschscholzia californica cells produces coptisine and dehydrocheilanthifoline from pathway intermediates cheilanthifoline and stylopine, overview Coptis japonica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.8 ? x * 57748, sequence calculation Coptis japonica

Synonyms

EC Number Synonyms Comment Organism
1.3.3.8 (S)-tetrahydroberberine oxidase
-
 Coptis japonica
1.3.3.8 THBO
-
 Coptis japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.8 30
-
 assay at Coptis japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.8 7
-
 assay at Coptis japonica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.3.3.8 Coptis japonica sequence calculation
-
 6.1

General Information

EC Number General Information Comment Organism
1.3.3.8 metabolism (S)-tetrahydroberberine oxidase is the enzyme in the last step of berberine biosynthesis Coptis japonica