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Literature summary extracted from

  • Grünweller, S.; Kesselmeier, J.
    Characterization of a membrane bound beta-glucosidase responsible for the activation of oat leaf saponins (1985), Phytochemistry, 24, 1941-1943.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.188 Hg2+ complete inhibition at 1 mM Avena sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.188 0.183
-
 avenacoside B at pH 6.5 and 25°C Avena sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.188 tonoplast bound to the tonoplast membrane Avena sativa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.188 additional information not influenced by 1 mM Cu2+ Avena sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.188 68000
-
 x * 68000, SDS-PAGE Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.188 avenacoside A + H2O Avena sativa
-
 26-desgluco-avenacoside A + D-glucose
-
 ?
3.2.1.188 avenacoside B + H2O Avena sativa
-
 26-desgluco-avenacoside B + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.188 Avena sativa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.188 leaf
-
 Avena sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.188 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Avena sativa 4-nitrophenol + D-glucose
-
 ?
3.2.1.188 avenacoside A + H2O
-
 Avena sativa 26-desgluco-avenacoside A + D-glucose
-
 ?
3.2.1.188 avenacoside B + H2O
-
 Avena sativa 26-desgluco-avenacoside B + D-glucose
-
 ?
3.2.1.188 additional information no activity with hederacoside C and cellobiose Avena sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.188 ? x * 68000, SDS-PAGE Avena sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.188 40
-
-
 Avena sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.188 6 7
-
 Avena sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.188 4.8 8 50% activity at pH 4.8 and 8.0, respectively Avena sativa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.188 0.5
-
 at pH 6.5 and 25°C Avena sativa Hg2+