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Literature summary extracted from

  • Reimann, J.; Esser, D.; Orell, A.; Amman, F.; Pham, T.K.; Noirel, J.; Lindas A.C.; Bernander, R.; Wright, P.C.; Siebers, B.; Albers, S.V.
    Archaeal signal transduction: impact of protein phosphatase deletions on cell size, motility, and energy metabolism in Sulfolobus acidocaldarius (2013), Mol. Cell. Proteomics, 12, 3908-3923.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 expression in Escherichia coli Rosetta (DE3)-RIL Sulfolobus acidocaldarius
3.1.3.48 expression in Escherichia coli Rosetta (DE3)-RIL Sulfolobus acidocaldarius

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.16 okadaic acid 56% activity in presence of 10 nM okadaic acid Sulfolobus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.16 0.0536
-
 RRA(pT)VA pH 6.5, 70°C Sulfolobus acidocaldarius
3.1.3.16 0.423
-
 NIDAIRA(pS)LNIMSR pH 6.5, 70°C Sulfolobus acidocaldarius
3.1.3.16 0.49
-
 ETTYERW(pT)TITQRER pH 6.5, 70°C Sulfolobus acidocaldarius
3.1.3.16 0.53
-
 4-nitrophenyl phosphate pH 6.5, 70°C Sulfolobus acidocaldarius
3.1.3.16 2.5
-
 4-nitrophenyl phosphate pH 6.5, 70°C Sulfolobus acidocaldarius
3.1.3.48 0.53
-
 4-nitrophenyl phosphate pH 6.5, 70°C Sulfolobus acidocaldarius
3.1.3.48 0.53
-
 TEVGKRI(pY)RLVGDKN pH 6.5, 70°C Sulfolobus acidocaldarius

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 Cd2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius
3.1.3.16 Co2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius
3.1.3.16 Mg2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius
3.1.3.16 Mn2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius
3.1.3.16 Ni2+ the enzyme requires a divalent metal ion for activity. The highest activity is observed with Cu2+, followed by Mn2+, Ni2+, Mg2+, Cd2+and Co2+, in the presence of EGTA. When EGTA is replaced by EDTA, activity can be observed. In the absence of divalent metal ions the enzyme is inactive Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.16 [protein]-L-serine phosphate + H2O Sulfolobus acidocaldarius
-
 [protein]-L-serine + phosphate
-
 ?
3.1.3.16 [protein]-L-serine phosphate + H2O Sulfolobus acidocaldarius MW001
-
 [protein]-L-serine + phosphate
-
 ?
3.1.3.16 [protein]-L-threonine phosphate + H2O Sulfolobus acidocaldarius
-
 [protein]-L-threonine + phosphate
-
 ?
3.1.3.48 [protein]-L-tyrosine phosphate + H2O Sulfolobus acidocaldarius
-
 [protein]-L-tyrosine + phosphate
-
 ?
3.1.3.48 [protein]-L-tyrosine phosphate + H2O Sulfolobus acidocaldarius MW001
-
 [protein]-L-tyrosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Sulfolobus acidocaldarius Q4JAD1 the wild type strain DSM 639 missing 322 bp called MW001
-
3.1.3.16 Sulfolobus acidocaldarius Q4JB88 the wild type strain DSM 639 missing 322 bp called MW001
-
3.1.3.16 Sulfolobus acidocaldarius ATCC 33909 Q4JAD1 the wild type strain DSM 639 missing 322 bp called MW001
-
3.1.3.16 Sulfolobus acidocaldarius MW001 Q4JB88 the wild type strain DSM 639 missing 322 bp called MW001
-
3.1.3.48 Sulfolobus acidocaldarius Q4JB88 the wild type strain DSM 639 missing 322 bp called MW001
-
3.1.3.48 Sulfolobus acidocaldarius MW001 Q4JB88 the wild type strain DSM 639 missing 322 bp called MW001
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16
-
 Sulfolobus acidocaldarius
3.1.3.48
-
 Sulfolobus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 4-nitrophenyl phosphate + H2O
-
 Sulfolobus acidocaldarius 4-nitrophenol + phosphate
-
 ?
3.1.3.16 4-nitrophenyl phosphate + H2O
-
 Sulfolobus acidocaldarius MW001 4-nitrophenol + phosphate
-
 ?
3.1.3.16 4-nitrophenyl phosphate + H2O
-
 Sulfolobus acidocaldarius ATCC 33909 4-nitrophenol + phosphate
-
 ?
3.1.3.16 ETTYERW(pT)TITQRER + H2O
-
 Sulfolobus acidocaldarius ETTYERWTTITQRER + phosphate
-
 ?
3.1.3.16 additional information no activity with TEVGKRI(pY)RLVGDKN Sulfolobus acidocaldarius ?
-
 ?
3.1.3.16 additional information no activity with TEVGKRI(pY)RLVGDKN Sulfolobus acidocaldarius ATCC 33909 ?
-
 ?
3.1.3.16 NIDAIRA(pS)LNIMSR + H2O
-
 Sulfolobus acidocaldarius NIDAIRASLNIMSR + phosphate
-
 ?
3.1.3.16 RRA(pT)VA + H2O
-
 Sulfolobus acidocaldarius RRATVA + phosphate
-
 ?
3.1.3.16 RRA(pT)VA + H2O
-
 Sulfolobus acidocaldarius ATCC 33909 RRATVA + phosphate
-
 ?
3.1.3.16 [protein]-L-serine phosphate + H2O
-
 Sulfolobus acidocaldarius [protein]-L-serine + phosphate
-
 ?
3.1.3.16 [protein]-L-serine phosphate + H2O
-
 Sulfolobus acidocaldarius MW001 [protein]-L-serine + phosphate
-
 ?
3.1.3.16 [protein]-L-threonine phosphate + H2O
-
 Sulfolobus acidocaldarius [protein]-L-threonine + phosphate
-
 ?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Sulfolobus acidocaldarius 4-nitrophenol + phosphate
-
 ?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Sulfolobus acidocaldarius MW001 4-nitrophenol + phosphate
-
 ?
3.1.3.48 TEVGKRI(pY)RLVGDKN + H2O
-
 Sulfolobus acidocaldarius TEVGKRIYRLVGDKN + phosphate
-
 ?
3.1.3.48 TEVGKRI(pY)RLVGDKN + H2O
-
 Sulfolobus acidocaldarius MW001 TEVGKRIYRLVGDKN + phosphate
-
 ?
3.1.3.48 [protein]-L-tyrosine phosphate + H2O
-
 Sulfolobus acidocaldarius [protein]-L-tyrosine + phosphate
-
 ?
3.1.3.48 [protein]-L-tyrosine phosphate + H2O
-
 Sulfolobus acidocaldarius MW001 [protein]-L-tyrosine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 Saci-PP2A
-
 Sulfolobus acidocaldarius
3.1.3.16 Saci-PTP dual specific phosphatase EC 3.1.3.16/EC 3.1.3.48 Sulfolobus acidocaldarius
3.1.3.16 Saci0545 locus name, dual specific phosphatase EC 3.1.3.16/EC 3.1.3.48 Sulfolobus acidocaldarius
3.1.3.16 Saci0884
-
 Sulfolobus acidocaldarius
3.1.3.48 Saci-PTP dual specific phosphatase EC 3.1.3.16/EC 3.1.3.48 Sulfolobus acidocaldarius
3.1.3.48 Saci0545 locus name, dual specific phosphatase EC 3.1.3.16/EC 3.1.3.48 Sulfolobus acidocaldarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.16 70
-
 assay at Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.16 6.5
-
 assay at Sulfolobus acidocaldarius