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Literature summary extracted from

  • Li, M.; Gan, Z.; Cui, Y.; Shi, C.; Shi, X.
    Structure and function characterization of the phytoene desaturase related to the lutein biosynthesis in Chlorella protothecoides CS-41 (2013), Mol. Biol. Rep., 40, 3351-3361.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.29 gene pds, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Escherichia coli strain JM109 or BL21(DE3), subcloning in Escherichia coli strain DH5alpha Auxenochlorella protothecoides

Protein Variants

EC Number Protein Variants Comment Organism
1.3.99.29 N144D random and site-directed mutagenesis, inactive mutant Auxenochlorella protothecoides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.29 61000
-
 x * 61000, recombinant enzyme, SDS-PAGE Auxenochlorella protothecoides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.29 15-cis-phytoene + acceptor Auxenochlorella protothecoides
-
 all-trans-phytofluene + reduced acceptor
-
 ?
1.3.99.29 15-cis-phytoene + acceptor Auxenochlorella protothecoides CS-41
-
 all-trans-phytofluene + reduced acceptor
-
 ?
1.3.99.29 all-trans-phytofluene + acceptor Auxenochlorella protothecoides
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.29 all-trans-phytofluene + acceptor Auxenochlorella protothecoides CS-41
-
 all-trans-zeta-carotene + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.29 Auxenochlorella protothecoides G8Z936 gene pds
-
1.3.99.29 Auxenochlorella protothecoides CS-41 G8Z936 gene pds
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.29 15-cis-phytoene + acceptor
-
 Auxenochlorella protothecoides all-trans-phytofluene + reduced acceptor
-
 ?
1.3.99.29 15-cis-phytoene + acceptor
-
 Auxenochlorella protothecoides CS-41 all-trans-phytofluene + reduced acceptor
-
 ?
1.3.99.29 all-trans-phytofluene + acceptor
-
 Auxenochlorella protothecoides all-trans-zeta-carotene + reduced acceptor
-
 ?
1.3.99.29 all-trans-phytofluene + acceptor
-
 Auxenochlorella protothecoides CS-41 all-trans-zeta-carotene + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.29 ? x * 61000, recombinant enzyme, SDS-PAGE Auxenochlorella protothecoides

Synonyms

EC Number Synonyms Comment Organism
1.3.99.29 PDS
-
 Auxenochlorella protothecoides
1.3.99.29 phytoene desaturase
-
 Auxenochlorella protothecoides

General Information

EC Number General Information Comment Organism
1.3.99.29 additional information amino acid residue 144 is crutial to the activity of the PDS enzyme Auxenochlorella protothecoides