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Literature summary extracted from

  • Banerjee, N.; Bhattacharyya, D.
    UDP-glucose dehydrogenase from Capra hircus liver: Purification, partial characterization and evaluation as a coupling enzyme in UDP-galactose 4-epimerase assay (2011), J. Mol. Catal. B, 68, 37-43.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.22 NADH 0.05 mM, partial inhibition Capra hircus
1.1.1.22 UDP 0.5 mM, partial inhibition Capra hircus
1.1.1.22 UDP-alpha-D-glucuronate 0.05 mM, partial inhibition Capra hircus
1.1.1.22 UDP-galactose 0.5 mM, partial inhibition Capra hircus
1.1.1.22 UDP-xylose 0.05 mM, partial inhibition Capra hircus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.22 0.021
-
 UDP-alpha-D-glucose pH 8.8, 25°C Capra hircus
1.1.1.22 0.132
-
 NAD+ pH 8.8, 25°C Capra hircus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.22 50000
-
 6 * 50000, SDS-PAGE Capra hircus
1.1.1.22 300000
-
 gel filtration Capra hircus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.22 Capra hircus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.22
-
 Capra hircus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.22 liver
-
 Capra hircus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.22 342.9
-
 pH 8.8, 25°C Capra hircus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.22 additional information no substrates: UDP-D-galactose, UTP, 5'-UMP and D-galactose Capra hircus ?
-
 ?
1.1.1.22 UDP-alpha-D-glucose + 2 NAD+ + H2O
-
 Capra hircus UDP-alpha-D-glucuronate + 2 NADH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.22 hexamer 6 * 50000, SDS-PAGE Capra hircus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.22 25 45
-
 Capra hircus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.22 8.4
-
-
 Capra hircus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.22 8.8
-
 80-83% of maximum activity Capra hircus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.22 5.4 6.5 enzyme retains 93-97% of initial activity Capra hircus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.22 NAD+
-
 Capra hircus
1.1.1.22 NADP+ about 5-12% of the activity with NAD+ Capra hircus