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Literature summary extracted from

  • Cao, L.; Gao, Y.; Wu, G.; Li, M.; Xu, J.; He, J.; Li, S.; Hong, Q.
    Cloning of three 2,3-dihydroxybiphenyl-1,2-dioxygenase genes from Achromobacter sp. BP3 and the analysis of their roles in the biodegradation of biphenyl (2013), J. Hazard. Mater., 261, 246-252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 gene bphC1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 Achromobacter sp.
1.13.11.39 gene bphC2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 Achromobacter sp.
1.13.11.39 gene bphC3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression in in Escherichia coli strain BL21 Achromobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.39 Ca2+
-
 Achromobacter sp.
1.13.11.39 Co2+
-
 Achromobacter sp.
1.13.11.39 Cu2+
-
 Achromobacter sp.
1.13.11.39 Fe2+
-
 Achromobacter sp.
1.13.11.39 Fe3+ slight inhibition Achromobacter sp.
1.13.11.39 Mg2+
-
 Achromobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 0.0021
-
 3-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.00213
-
 biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.0049
-
 biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.0062
-
 catechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.01672
-
 catechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.0273
-
 4-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.0391
-
 biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.0616
-
 3-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.0976
-
 catechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.1306
-
 3-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.2824
-
 4-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 0.3605
-
 4-methylcatechol pH 7.5, 30°C Achromobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Fe2+ activates 270% Achromobacter sp.
1.13.11.39 additional information no effect by Fe3+, Mg2+ and Ca2+ Achromobacter sp.
1.13.11.39 additional information no effect by Fe3+, Mg2+ and Ca2+, poor activating effect by Fe2+ Achromobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.39 biphenyl-2,3-diol + O2 Achromobacter sp.
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 Achromobacter sp. BP3
-
 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Achromobacter sp.
-
 isolated from petroleum-contaminated soil, gene bphC3
-
1.13.11.39 Achromobacter sp. B3VTK1 BphC1; isolated from petroleum-contaminated soil, gene bphC1
-
1.13.11.39 Achromobacter sp. B3VTL5 BphC2; isolated from petroleum-contaminated soil, gene bphC2
-
1.13.11.39 Achromobacter sp. BP3
-
 isolated from petroleum-contaminated soil, gene bphC3
-
1.13.11.39 Achromobacter sp. BP3 B3VTK1 BphC1; isolated from petroleum-contaminated soil, gene bphC1
-
1.13.11.39 Achromobacter sp. BP3 B3VTL5 BphC2; isolated from petroleum-contaminated soil, gene bphC2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 3-methylcatechol + O2
-
 Achromobacter sp. ?
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Achromobacter sp. BP3 ?
-
 ?
1.13.11.39 4-methylcatechol + O2
-
 Achromobacter sp. ?
-
 ?
1.13.11.39 4-methylcatechol + O2
-
 Achromobacter sp. BP3 ?
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Achromobacter sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 highly preferred substrate Achromobacter sp. 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2
-
 Achromobacter sp. BP3 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 biphenyl-2,3-diol + O2 highly preferred substrate Achromobacter sp. BP3 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 catechol + O2
-
 Achromobacter sp. cis,cis-muconate
-
 ?
1.13.11.39 catechol + O2
-
 Achromobacter sp. BP3 cis,cis-muconate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 2,3-dihydroxybiphenyl 1,2-dioxygenase
-
 Achromobacter sp.
1.13.11.39 BphC
-
 Achromobacter sp.
1.13.11.39 BphC1
-
 Achromobacter sp.
1.13.11.39 BphC2
-
 Achromobacter sp.
1.13.11.39 BphC3
-
 Achromobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.39 30
-
-
 Achromobacter sp.
1.13.11.39 40
-
-
 Achromobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.39 10 60 activity range, profile overview Achromobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.39 1.19
-
 4-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 3.25
-
 4-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 7.36
-
 catechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 8.78
-
 biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 9.87
-
 catechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 10.15
-
 3-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 12.02
-
 3-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 21.63
-
 3-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 43.2
-
 4-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 52.56
-
 catechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 53.83
-
 biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 86.32
-
 biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.39 8
-
-
 Achromobacter sp.
1.13.11.39 8 9
-
 Achromobacter sp.
1.13.11.39 9
-
-
 Achromobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.39 5 10 activity range, profile overview Achromobacter sp.

Expression

EC Number Organism Comment Expression
1.13.11.39 Achromobacter sp. gene bphC2 is constitutively expressed in strain BP3 up
1.13.11.39 Achromobacter sp. gene bphC3 is induced by both biphenyl and toluene up
1.13.11.39 Achromobacter sp. the bphC1 gene is only induced by biphenyl up

General Information

EC Number General Information Comment Organism
1.13.11.39 metabolism BphC1 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview Achromobacter sp.
1.13.11.39 metabolism BphC2 might play a supplementary role and contribute more to the upstream than to the downstream pathway of biphenyl, overview Achromobacter sp.
1.13.11.39 metabolism BphC3 plays a role in the upstream and downstream metabolic pathways of biphenyl, overview Achromobacter sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.39 4.21
-
 4-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 9
-
 4-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 92
-
 3-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 101.1
-
 catechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 164.8
-
 3-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 224.6
-
 biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 440.2
-
 catechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 1582
-
 4-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 8477
-
 catechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 10300
-
 3-methylcatechol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 10990
-
 biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.
1.13.11.39 40530
-
 biphenyl-2,3-diol pH 7.5, 30°C Achromobacter sp.