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Literature summary extracted from

  • Mohammadi, H.S.; Omidinia, E.
    Process integration for the recovery and purification of recombinant Pseudomonas fluorescens proline dehydrogenase using aqueous two-phase systems (2013), J. Chromatogr. B, 929, 11-17.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.99.B2 expressed in Escherichia coli BL21 (DE3) pLysS cells Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.99.B2 40000
-
 x * 40000, SDS-PAGE Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.B2 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.B2
-
 Pseudomonas fluorescens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.5.99.B2
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.B2 L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
-
 Pseudomonas fluorescens DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.99.B2 ? x * 40000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
1.5.99.B2 PRODH
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.B2 FAD
-
 Pseudomonas fluorescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.99.B2 Pseudomonas fluorescens isoelectric focusing
-
 7