EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.7.1 | biotechnology | pyruvate:ferredoxin oxidoreductase PFR1 and [Fe-Fe]-hydrogenase HYDA1 of Chlamydomonas can be coupled for pyruvate-dependent H2 production | Chlamydomonas reinhardtii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.7.1 | expressed in Escherichia coli BL21(DE3) cells | Chlamydomonas reinhardtii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.7.1 | 0.67 | - |
CoA | in 100 mM potassium phosphate buffer, pH 6.8, temperature not specified in the publication | Chlamydomonas reinhardtii | |
1.2.7.1 | 1.65 | - |
pyruvate | in 100 mM potassium phosphate buffer, pH 6.8, temperature not specified in the publication | Chlamydomonas reinhardtii | |
1.2.7.1 | 2.33 | - |
oxidized methyl viologen | in 100 mM potassium phosphate buffer, pH 6.8, temperature not specified in the publication | Chlamydomonas reinhardtii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.7.1 | 144000 | - |
x * 144000, SDS-PAGE | Chlamydomonas reinhardtii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.1 | additional information | Chlamydomonas reinhardtii | the enzyme cannot use 2-oxoglutarate as substrate | ? | - |
? | |
1.2.7.1 | additional information | Chlamydomonas reinhardtii | the enzyme is able to transfer electrons from pyruvate to the [Fe-Fe]-hydrogenase HYDA1, using the ferredoxins PETF and FDX2 as electron carriers | ? | - |
? | |
1.2.7.1 | additional information | Chlamydomonas reinhardtii CC124 | the enzyme cannot use 2-oxoglutarate as substrate | ? | - |
? | |
1.2.7.1 | additional information | Chlamydomonas reinhardtii CC124 | the enzyme is able to transfer electrons from pyruvate to the [Fe-Fe]-hydrogenase HYDA1, using the ferredoxins PETF and FDX2 as electron carriers | ? | - |
? | |
1.2.7.1 | pyruvate + CoA + oxidized ferredoxin | Chlamydomonas reinhardtii | - |
acetyl-CoA + CO2 + reduced ferredoxin + H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + oxidized ferredoxin | Chlamydomonas reinhardtii CC124 | - |
acetyl-CoA + CO2 + reduced ferredoxin + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.7.1 | Chlamydomonas reinhardtii | - |
- |
- |
1.2.7.1 | Chlamydomonas reinhardtii CC124 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.7.1 | Ni-NTA column chromatography | Chlamydomonas reinhardtii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.7.1 | additional information | the enzyme cannot use 2-oxoglutarate as substrate | Chlamydomonas reinhardtii | ? | - |
? | |
1.2.7.1 | additional information | the enzyme is able to transfer electrons from pyruvate to the [Fe-Fe]-hydrogenase HYDA1, using the ferredoxins PETF and FDX2 as electron carriers | Chlamydomonas reinhardtii | ? | - |
? | |
1.2.7.1 | additional information | the enzyme cannot use 2-oxoglutarate as substrate | Chlamydomonas reinhardtii CC124 | ? | - |
? | |
1.2.7.1 | additional information | the enzyme is able to transfer electrons from pyruvate to the [Fe-Fe]-hydrogenase HYDA1, using the ferredoxins PETF and FDX2 as electron carriers | Chlamydomonas reinhardtii CC124 | ? | - |
? | |
1.2.7.1 | oxaloacetate + CoA + oxidized methyl viologen | - |
Chlamydomonas reinhardtii | ? + reduced methyl viologen + H+ | - |
? | |
1.2.7.1 | oxaloacetate + CoA + oxidized methyl viologen | - |
Chlamydomonas reinhardtii CC124 | ? + reduced methyl viologen + H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + oxidized ferredoxin | - |
Chlamydomonas reinhardtii | acetyl-CoA + CO2 + reduced ferredoxin + H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + oxidized ferredoxin | - |
Chlamydomonas reinhardtii CC124 | acetyl-CoA + CO2 + reduced ferredoxin + H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + oxidized methyl viologen | - |
Chlamydomonas reinhardtii | acetyl-CoA + CO2 + reduced methyl viologen + H+ | - |
? | |
1.2.7.1 | pyruvate + CoA + oxidized methyl viologen | - |
Chlamydomonas reinhardtii CC124 | acetyl-CoA + CO2 + reduced methyl viologen + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.7.1 | ? | x * 144000, SDS-PAGE | Chlamydomonas reinhardtii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.7.1 | PFOR | - |
Chlamydomonas reinhardtii |
1.2.7.1 | PFR1 | - |
Chlamydomonas reinhardtii |
1.2.7.1 | pyruvate:ferredoxin oxidoreductase | - |
Chlamydomonas reinhardtii |