Literature summary extracted from

Komori, K.; Miyata, T.; Daiyasu, H.; Toh, H.; Shinagawa, H.; Ishino, aY.
Domain analysis of an archaeal RadA protein for the strand exchange activity (2000), J. Biol. Chem., 275, 33791-33797.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.6.4.B7	expression in Escherichia coli	Pyrococcus furiosus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.6.4.B7	Pyrococcus furiosus	O74036	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.6.4.B7	ATP + H2O	the central core domain of RadA is essential for the strand exchange activity and that the N-terminal domain contributes to the enhancement of the reaction efficiency	Pyrococcus furiosus	ADP + phosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.6.4.B7	oligomer	stable selfassociation of the RadA proteins	Pyrococcus furiosus

Synonyms

EC Number	Synonyms	Comment	Organism
3.6.4.B7	RadA	-	Pyrococcus furiosus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.6.4.B7	70	-	assay at	Pyrococcus furiosus

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.6.4.B7	0.0017	-	ATP	pH 7.5, 70°C	Pyrococcus furiosus
3.6.4.B7	0.017	-	ATP	pH 7.5, 70°C, addition of dsDNA	Pyrococcus furiosus
3.6.4.B7	0.0345	-	ATP	pH 7.5, 70°C, addition of ssDNA	Pyrococcus furiosus

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Release 2023.1 (January 2023)