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Literature summary extracted from

  • Rusnak, P.; Haney, P.; Konisky, J.
    The adenylate kinases from a mesophilic and three thermophilic methanogenic members of the archaea (1995), J. Bacteriol., 177, 2977-2981.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.4.3 enzyme is fully active in air Methanococcus voltae
2.7.4.3 enzyme is fully active in air Methanotorris igneus
2.7.4.3 enzyme is fully active in air Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.3 AMP 1 mM, complete inhibition of ADP-dependent ATP production Methanococcus voltae
2.7.4.3 P1,P5-di(adenosine-5')pentaphosphate
-
 Methanocaldococcus jannaschii
2.7.4.3 P1,P5-di(adenosine-5')pentaphosphate 50% inhibition at 50 mM, whether assayed in the direction of ATP formation from ADP or of ATP conversion to ADP Methanococcus voltae
2.7.4.3 P1,P5-di(adenosine-5')pentaphosphate
-
 Methanothermococcus thermolithotrophicus
2.7.4.3 P1,P5-di(adenosine-5')pentaphosphate
-
 Methanotorris igneus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.3 cytosol
-
 Methanothermococcus thermolithotrophicus 5829
-
2.7.4.3 cytosol
-
 Methanococcus voltae 5829
-
2.7.4.3 cytosol
-
 Methanotorris igneus 5829
-
2.7.4.3 cytosol
-
 Methanocaldococcus jannaschii 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.3 K+ maximum stimulation at 100 mM Methanococcus voltae
2.7.4.3 K+ maximum stimulation at 100 mM Methanocaldococcus jannaschii
2.7.4.3 K+ maximum stimulation at 200 mM Methanotorris igneus
2.7.4.3 K+ maximum stimulation at 400 mM Methanothermococcus thermolithotrophicus
2.7.4.3 Mg2+ activity is Mg2+ dependent Methanococcus voltae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.3 25000
-
 x * 25000, SDS-PAGE Methanococcus voltae
2.7.4.3 25000
-
 x * 25000 (approximately), SDS-PAGE Methanothermococcus thermolithotrophicus
2.7.4.3 25000
-
 x * 25000 (approximately), SDS-PAGE Methanotorris igneus
2.7.4.3 25000
-
 x * 25000 (approximately), SDS-PAGE Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Methanocaldococcus jannaschii P43409
-
-
2.7.4.3 Methanocaldococcus jannaschii DSM 2661 P43409
-
-
2.7.4.3 Methanococcus voltae P43411
-
-
2.7.4.3 Methanothermococcus thermolithotrophicus P43410
-
-
2.7.4.3 Methanotorris igneus P43408
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.3
-
 Methanothermococcus thermolithotrophicus
2.7.4.3
-
 Methanococcus voltae
2.7.4.3
-
 Methanotorris igneus
2.7.4.3
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.3 14
-
 substrate: ATP, pH and temperature not specified in the publication Methanococcus voltae
2.7.4.3 56
-
 substrate: ATP, pH and temperature not specified in the publication Methanotorris igneus
2.7.4.3 81
-
 substrate: ATP, pH and temperature not specified in the publication Methanothermococcus thermolithotrophicus
2.7.4.3 89
-
 substrate: ATP, pH and temperature not specified in the publication Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ATP + AMP
-
 Methanothermococcus thermolithotrophicus 2 ADP
-
 r
2.7.4.3 ATP + AMP
-
 Methanococcus voltae 2 ADP
-
 r
2.7.4.3 ATP + AMP
-
 Methanotorris igneus 2 ADP
-
 r
2.7.4.3 ATP + AMP
-
 Methanocaldococcus jannaschii 2 ADP
-
 r
2.7.4.3 ATP + AMP
-
 Methanocaldococcus jannaschii DSM 2661 2 ADP
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.4.3 ? x * 25000, SDS-PAGE Methanococcus voltae
2.7.4.3 ? x * 25000 (approximately), SDS-PAGE Methanothermococcus thermolithotrophicus
2.7.4.3 ? x * 25000 (approximately), SDS-PAGE Methanotorris igneus
2.7.4.3 ? x * 25000 (approximately), SDS-PAGE Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.3 30
-
-
 Methanococcus voltae
2.7.4.3 70
-
-
 Methanothermococcus thermolithotrophicus
2.7.4.3 70
-
-
 Methanotorris igneus
2.7.4.3 80 90
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.3 40
-
 1 h, the enzyme maintains 77.5% residual activity compared with the enzyme stored on ice Methanococcus voltae
2.7.4.3 59
-
 1 h, the enzyme maintains 47% residual activity compared with the enzyme stored on ice Methanococcus voltae
2.7.4.3 68
-
 t1/2: 30 min Methanocaldococcus jannaschii
2.7.4.3 68
-
 t1/2: 4 min Methanococcus voltae
2.7.4.3 68
-
 t1/2: 25 min Methanothermococcus thermolithotrophicus
2.7.4.3 68
-
 t1/2: 43 min Methanotorris igneus
2.7.4.3 89
-
 t1/2: 1.5 min Methanococcus voltae
2.7.4.3 89
-
 t1/2: 17 min Methanotorris igneus
2.7.4.3 89
-
 t1/2: 6.0 min Methanothermococcus thermolithotrophicus
2.7.4.3 89
-
 t1/2: 6.0 min Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.3 6 10 enzyme shows similar activity over a pH range from 6 to 10 Methanococcus voltae