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Literature summary extracted from
- Regiospecific chlorination of (S)-beta-tyrosyl-S-carrier protein catalyzed by SgcC3 in the biosynthesis of the enediyne antitumor antibiotic C-1027 (2007), J. Am. Chem. Soc., 129, 12432-12438.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.15 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces globisporus |
| 1.14.19.B8 | - |
Streptomyces globisporus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.15 | (3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl-[peptidyl-carrier protein SgcC2] + FADH2 + O2 | Streptomyces globisporus | - |
(3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl-[peptidyl-carrier protein SgcC2] + FAD + H2O | - |
? |  |
| 1.14.14.15 | additional information | Streptomyces globisporus | SgcC3 also efficiently catalyzes bromination but not fluorination or iodination | ? | - |
? | |
| 1.14.19.B8 | S-(3S)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Cl- + O2 | Streptomyces globisporus | the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027 | S-(S3)-3-chloro-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.15 | Streptomyces globisporus | - |
- |
- |
| 1.14.19.B8 | Streptomyces globisporus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.15 | Ni-NTA agarose column chromatography | Streptomyces globisporus |
| 1.14.19.B8 | - |
Streptomyces globisporus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.15 | (3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl-[peptidyl-carrier protein SgcC2] + FADH2 + O2 | - |
Streptomyces globisporus | (3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl-[peptidyl-carrier protein SgcC2] + FAD + H2O | - |
? | |
| 1.14.14.15 | (R)-alpha-tyrosyl-[SgcC2] + FADH2 + O2 | SgcC3 can utilize both (S)- and (R)-alpha-tyrosyl-[SgcC2] but not 3-hydroxy-alpha-tyrosyl-[SgcC2] as a substrate | Streptomyces globisporus | ? | - |
? | |
| 1.14.14.15 | (S)-alpha-tyrosyl-[SgcC2] + FADH2 + O2 | SgcC3 can utilize both (S)- and (R)-alpha-tyrosyl-[SgcC2] but not 3-hydroxy-alpha-tyrosyl-[SgcC2] as a substrate | Streptomyces globisporus | ? | - |
? | |
| 1.14.14.15 | additional information | SgcC3 also efficiently catalyzes bromination but not fluorination or iodination | Streptomyces globisporus | ? | - |
? | |
| 1.14.14.15 | additional information | no activity with 3-hydroxy-alpha-tyrosyl-S-SgcC2 | Streptomyces globisporus | ? | - |
? | |
| 1.14.19.B8 | additional information | the enzyme does not catalyze fluorination or iodination. The enzyme can not utilize 3-hydroxy-beta-tyrosyl-S-SgcC2 as a substrate | Streptomyces globisporus | ? | - |
? | |
| 1.14.19.B8 | S-(3R)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Cl- + O2 | - |
Streptomyces globisporus | S-(3R)-3-chloro-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O | - |
? | |
| 1.14.19.B8 | S-(3S)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Br- + O2 | the enzyme can also efficiently catalyzes bromination but not fluorination or iodination | Streptomyces globisporus | S-(3S)-3-bromo-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O | - |
? | |
| 1.14.19.B8 | S-(3S)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Cl- + O2 | the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027 | Streptomyces globisporus | S-(S3)-3-chloro-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O | - |
? | |
| 1.14.19.B8 | S-(3S)-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + 2 FADH2 + 2 H+ + Cl- + O2 | - |
Streptomyces globisporus | S-(3S)-3-chloro-beta-tyrosyl-[peptidyl-carrier-protein SgcC2]-L-cysteine + FAD + 2 H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.15 | SgcC3 | - |
Streptomyces globisporus |
| 1.14.19.B8 | SgcC3 | - |
Streptomyces globisporus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.B8 | 37 | - |
assay at | Streptomyces globisporus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.B8 | 6 | - |
- |
Streptomyces globisporus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.B8 | 5.5 | 7.5 | pH 5.5: about 15% of maximal activity, pH 7.5: about 65% of maximal activity | Streptomyces globisporus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.15 | FAD | dependent on | Streptomyces globisporus | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.19.B8 | physiological function | the enzyme is involved in the biosynthesis of the (S)-3-chloro-5-hydroxy-beta-tyrosine moiety prior to incorporation into the chromoprotein antitumor antibiotic C-1027 | Streptomyces globisporus |
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