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Literature summary extracted from

  • Shin, K.C.; Nam, H.K.; Oh, D.K.
    Hydrolysis of flavanone glycosides by beta-glucosidase from Pyrococcus furiosus and its application to the production of flavanone aglycones from citrus extracts. (2013), J. Agric. Food Chem., 61, 11532-11540.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.B28 additional information the enzyme converts flavanone glycoside to flavanone aglycone via a one-step reaction. It may be useful in the production of the flavanone aglycones naringenin and hesperetin from flavanone glycosides in citrus extracts Pyrococcus furiosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B28 expression in Escherichia coli Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B28 1-methyl-1-butanol 10% (v/v), about% inhibition Pyrococcus furiosus
3.2.1.B28 2-propanol 10% (v/v), about% inhibition Pyrococcus furiosus
3.2.1.B28 acetone 10% (v/v), about% inhibition Pyrococcus furiosus
3.2.1.B28 DMSO 10% (v/v), about% inhibition Pyrococcus furiosus
3.2.1.B28 ethanol 10% (v/v), about 50% inhibition Pyrococcus furiosus
3.2.1.B28 formate 10% (v/v), about% inhibition Pyrococcus furiosus
3.2.1.B28 Isopropanol 10% (v/v), about% inhibition Pyrococcus furiosus
3.2.1.B28 methanol 10% (v/v), about% inhibition Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B28 1.6
-
 hesperidin pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 1.7
-
 naringin pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 3.7
-
 narirutin pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 4.6
-
 neohesperidin pH 5.5, 95°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B28 55488
-
 x * 55488, calculated from sequence Pyrococcus furiosus
3.2.1.B28 56000
-
 x * 56000, SDS-PAGE Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B28 Pyrococcus furiosus Q51723
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B28 0.011
-
 substrate: rutin, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 0.081
-
 substrate: neoponcirin, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 0.085
-
 substrate: diosmin, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 0.091
-
 substrate: poncirin, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 0.102
-
 substrate naringin, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 0.103
-
 substrate: neohesperidin, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 0.153
-
 substrate: narirutin, pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 0.172
-
 substrate: hesperidin, pH 5.5, 95°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B28 diosmin + H2O
-
 Pyrococcus furiosus diosmetin + rutinose
-
 ?
3.2.1.B28 hesperidin + H2O
-
 Pyrococcus furiosus hesperetin + rutinose
-
 ?
3.2.1.B28 naringin + H2O
-
 Pyrococcus furiosus naringenin + rutinose
-
 ?
3.2.1.B28 narirutin + H2O
-
 Pyrococcus furiosus naringenin + neohesperidose
-
 ?
3.2.1.B28 neohesperidin + H2O
-
 Pyrococcus furiosus hesperetin + neohesperidose
-
 ?
3.2.1.B28 neoponcirin + H2O
-
 Pyrococcus furiosus isosakuranetin + rutinose
-
 ?
3.2.1.B28 poncirin + H2O
-
 Pyrococcus furiosus isosakuranetin + neohesperidose
-
 ?
3.2.1.B28 rutin + H2O
-
 Pyrococcus furiosus quercetin + rutinose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B28 ? x * 56000, SDS-PAGE Pyrococcus furiosus
3.2.1.B28 ? x * 55488, calculated from sequence Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B28 95
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B28 75 95 the activity increases from 75°C to 95°C, activity at 75°C is about 40% of the activity at 95°C, substrate: hesperidin Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B28 75
-
 half-life: 637 h Pyrococcus furiosus
3.2.1.B28 80
-
 half-life: 392 h Pyrococcus furiosus
3.2.1.B28 85
-
 half-life: 235 h Pyrococcus furiosus
3.2.1.B28 90
-
 half-life: 138 h Pyrococcus furiosus
3.2.1.B28 95
-
 half-life: 88 h Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B28 27.7
-
 naringin pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 68.4
-
 hesperidin pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 74.1
-
 narirutin pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 84.8
-
 neohesperidin pH 5.5, 95°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B28 5.5
-
 substrate: hesperidin Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B28 4.5 6.5 pH 4.5: about 50% of maximal activity, pH 6.5: about 60% of maximal activity, substrate: hesperidin Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B28 16.5
-
 naringin pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 18.5
-
 neohesperidin pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 19.8
-
 narirutin pH 5.5, 95°C Pyrococcus furiosus
3.2.1.B28 42.1
-
 hesperidin pH 5.5, 95°C Pyrococcus furiosus