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Literature summary extracted from

  • Evans, R.M.; Behiry, E.M.; Tey, L.H.; Guo, J.; Loveridge, E.J.; Allemann, R.K.
    Catalysis by dihydrofolate reductase from the psychropiezophile Moritella profunda (2010), ChemBioChem, 11, 2010-2017.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expressed in Escherichia coli BL21(DE3) cells Moritella profunda

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+ Moritella profunda
-
 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Moritella profunda
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 HiPrep 16/10 SP-XL column chromatography Moritella profunda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Moritella profunda 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 DHFR
-
 Moritella profunda

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 37.5
-
 the melting temperature of the apo-enzyme is 37.5°C and is not affected by pH over the range 5.0-9.0 Moritella profunda
1.5.1.3 40 50 the enzyme is able to perform its full catalytic rate for at least 1 h at temperatures up to 40°C. At higher temperatures the steady-state rate decreases rapidly with a half-life of inactivation of around 13 min at 45°C and 7 min at 50°C Moritella profunda

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 5.9
-
 7,8-dihydrofolate at pH 7.0 and 5°C Moritella profunda
1.5.1.3 8.2
-
 7,8-dihydrofolate at pH 7.0 and 10°C Moritella profunda
1.5.1.3 10.8
-
 7,8-dihydrofolate at pH 7.0 and 15°C Moritella profunda
1.5.1.3 14.8
-
 7,8-dihydrofolate at pH 7.0 and 20°C Moritella profunda
1.5.1.3 18.7
-
 7,8-dihydrofolate at pH 7.0 and 25°C Moritella profunda
1.5.1.3 25.3
-
 7,8-dihydrofolate at pH 7.0 and 30°C Moritella profunda
1.5.1.3 28.8
-
 7,8-dihydrofolate at pH 7.0 and 45°C Moritella profunda
1.5.1.3 32.5
-
 7,8-dihydrofolate at pH 7.0 and 35°C Moritella profunda
1.5.1.3 39.6
-
 7,8-dihydrofolate at pH 7.0 and 40°C Moritella profunda

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.3 6 8 the enzyme is most stable at pH 7.0, with a half-life for activity loss of around 13 days. Loss of activity is fastest at pH 5 with a half-life of 41 min, while the half-life of activity loss at pH 9.5 is about 2 h Moritella profunda

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Moritella profunda