EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.3.2 | expression in Trichoderma reesei | Aspergillus oryzae |
1.8.3.3 | expression in Trichoderma reesei | Aspergillus oryzae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.3.2 | KI | 1 mM, 65% residual activity | Aspergillus oryzae | |
1.8.3.2 | MgSO4 | 1 mM, 55% residual activity | Aspergillus oryzae | |
1.8.3.2 | Na2SO4 | 1 mM, 61% residual activity | Aspergillus oryzae | |
1.8.3.2 | sodium dodecylsulfate | 1 mM, 58% residual activity | Aspergillus oryzae | |
1.8.3.2 | Urea | 1 mM, 80% residual activity | Aspergillus oryzae | |
1.8.3.2 | ZnSO4 | 1 mM, complete inhibition | Aspergillus oryzae | |
1.8.3.3 | KI | 1 mM, 65% residual activity | Aspergillus oryzae | |
1.8.3.3 | MgSO4 | 1 mM, 55% residual activity | Aspergillus oryzae | |
1.8.3.3 | Na2SO4 | 1 mM, 61% residual activity | Aspergillus oryzae | |
1.8.3.3 | sodium dodecylsulfate | 1 mM, 58% residual activity | Aspergillus oryzae | |
1.8.3.3 | Urea | 1 mM, 80% residual activity | Aspergillus oryzae | |
1.8.3.3 | ZnSO4 | 1 mM, complete inhibition | Aspergillus oryzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.2 | 1.55 | - |
D-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 2.41 | - |
dithiothreitol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 2.78 | - |
glutathione | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 6.11 | - |
L-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 9.73 | - |
2-mercaptoethanol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 1.55 | - |
D-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 2.41 | - |
dithiothreitol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 2.78 | - |
glutathione | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 6.11 | - |
L-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 9.73 | - |
2-mercaptoethanol | pH 7.5, 20°C | Aspergillus oryzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.3.2 | 43959 | - |
x * 45000, SDS-PAGE, x * 43959, mass spectrometry | Aspergillus oryzae |
1.8.3.2 | 45000 | - |
x * 45000, SDS-PAGE, x * 43959, mass spectrometry | Aspergillus oryzae |
1.8.3.3 | 43959 | - |
x * 45000, SDS-PAGE, x * 43959, mass spectrometry | Aspergillus oryzae |
1.8.3.3 | 45000 | - |
x * 45000, SDS-PAGE, x * 43959, mass spectrometry | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.3.2 | Aspergillus oryzae | Q2UA33 | - |
- |
1.8.3.2 | Aspergillus oryzae ATCC 42149 | Q2UA33 | - |
- |
1.8.3.3 | Aspergillus oryzae | Q2UA33 | - |
- |
1.8.3.3 | Aspergillus oryzae ATCC 42149 | Q2UA33 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.8.3.2 | glycoprotein | sequence contains six potential N-glycosylation site. Deglycosylation reduces the molecluar mass to 40000 Da | Aspergillus oryzae |
1.8.3.3 | glycoprotein | sequence contains six potential N-glycosylation site. Deglycosylation reduces the molecluar mass to 40000 Da | Aspergillus oryzae |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.3.2 | recombinant protein | Aspergillus oryzae |
1.8.3.3 | recombinant protein | Aspergillus oryzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.3.2 | 2 2-mercaptoethanol + O2 | - |
Aspergillus oryzae | (ethyldisulfanyl)ethane + H2O2 | - |
? | |
1.8.3.2 | 2 2-mercaptoethanol + O2 | - |
Aspergillus oryzae ATCC 42149 | (ethyldisulfanyl)ethane + H2O2 | - |
? | |
1.8.3.2 | 2 D-Cys + O2 | - |
Aspergillus oryzae | D-cystine + H2O2 | - |
? | |
1.8.3.2 | 2 D-Cys + O2 | - |
Aspergillus oryzae ATCC 42149 | D-cystine + H2O2 | - |
? | |
1.8.3.2 | 2 dithiothreitol + O2 | - |
Aspergillus oryzae | dithiothreitol disulfide + H2O2 | - |
? | |
1.8.3.2 | 2 dithiothreitol + O2 | - |
Aspergillus oryzae ATCC 42149 | dithiothreitol disulfide + H2O2 | - |
? | |
1.8.3.2 | 2 glutathione + O2 | - |
Aspergillus oryzae | glutathione disulfide + H2O2 | - |
? | |
1.8.3.2 | 2 glutathione + O2 | - |
Aspergillus oryzae ATCC 42149 | glutathione disulfide + H2O2 | - |
? | |
1.8.3.2 | 2 L-Cys + O2 | - |
Aspergillus oryzae | L-cystine + H2O2 | - |
? | |
1.8.3.2 | 2 L-Cys + O2 | - |
Aspergillus oryzae ATCC 42149 | L-cystine + H2O2 | - |
? | |
1.8.3.3 | 2 2-mercaptoethanol + O2 | - |
Aspergillus oryzae | (ethyldisulfanyl)ethane + H2O2 | - |
? | |
1.8.3.3 | 2 D-Cys + O2 | - |
Aspergillus oryzae | D-cystine + H2O2 | - |
? | |
1.8.3.3 | 2 dithiothreitol + O2 | - |
Aspergillus oryzae | dithiothreitol disulfide + H2O2 | - |
? | |
1.8.3.3 | 2 glutathione + O2 | - |
Aspergillus oryzae | glutathione disulfide + H2O2 | - |
? | |
1.8.3.3 | 2 L-Cys + O2 | - |
Aspergillus oryzae | L-cystine + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.3.2 | ? | x * 45000, SDS-PAGE, x * 43959, mass spectrometry | Aspergillus oryzae |
1.8.3.3 | ? | x * 45000, SDS-PAGE, x * 43959, mass spectrometry | Aspergillus oryzae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.3.2 | Sox1 | - |
Aspergillus oryzae |
1.8.3.3 | Sox1 | - |
Aspergillus oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.3.2 | 40 | - |
24 h, more than 70% of initial activity | Aspergillus oryzae |
1.8.3.2 | 50 | - |
1 h, 40% of initial activity | Aspergillus oryzae |
1.8.3.2 | 57 | - |
melting tepmerature | Aspergillus oryzae |
1.8.3.3 | 40 | - |
24 h, more than 70% of initial activity | Aspergillus oryzae |
1.8.3.3 | 50 | - |
1 h, 40% of initial activity | Aspergillus oryzae |
1.8.3.3 | 57 | - |
melting temperature | Aspergillus oryzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.2 | 50 | 100 | 2-mercaptoethanol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 5950 | - |
D-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 52300 | - |
L-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 309000 | - |
dithiothreitol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 3600000 | - |
glutathione | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 50 | 100 | 2-mercaptoethanol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 5950 | - |
D-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 52300 | - |
L-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 309000 | - |
dithiothreitol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 3600000 | - |
glutathione | pH 7.5, 20°C | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.3.2 | 8 | - |
- |
Aspergillus oryzae |
1.8.3.3 | 8 | - |
- |
Aspergillus oryzae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.8.3.2 | 4 | 8 | 24 h, more than 80% of initial activity | Aspergillus oryzae |
1.8.3.3 | 4 | 8 | 24 h, more than 80% of initial activity | Aspergillus oryzae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.2 | 3840 | - |
D-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 8560 | - |
L-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 128000 | - |
dithiothreitol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 515000 | - |
2-mercaptoethanol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.2 | 1290000 | - |
glutathione | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 3840 | - |
D-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 8560 | - |
L-Cys | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 128000 | - |
dithiothreitol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 515000 | - |
2-mercaptoethanol | pH 7.5, 20°C | Aspergillus oryzae | |
1.8.3.3 | 1290000 | - |
glutathione | pH 7.5, 20°C | Aspergillus oryzae |