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Literature summary extracted from

  • Romero, E.; Fedkenheuer, M.; Chocklett, S,W.; Qi, J.; Oppenheimer, M.; Sobrado, P.
    Dual role of NADP(H) in the reaction of a flavin dependent N-hydroxylating monooxygenase (2012), Biochim. Biophys. Acta, 1824, 850-857.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.13.196 drug development essential for virulence, validating this enzyme as a drug target Aspergillus fumigatus

General Stability

EC Number General Stability Organism
1.14.13.196 NADP+, and not NAD+, protects the enzyme from proteolysis Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.195 0.012
-
 NADPH pH 7.5, 15°C Aspergillus fumigatus
1.14.13.195 0.1
-
 NADH pH 7.5, 15°C Aspergillus fumigatus
1.14.13.196 0.012
-
 NADPH pH 7.5, temperature not specified in the publication Aspergillus fumigatus
1.14.13.196 0.1
-
 NADH pH 7.5, temperature not specified in the publication Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.196 L-ornithine + NADH + H+ + O2 Aspergillus fumigatus first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
1.14.13.196 L-ornithine + NADPH + H+ + O2 Aspergillus fumigatus first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.195 Aspergillus fumigatus Q5SE95
-
-
1.14.13.196 Aspergillus fumigatus Q5SE95
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.196
-
 Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.195 L-ornithine + NADH + H+ + O2
-
 Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
1.14.13.195 L-ornithine + NADPH + H+ + O2
-
 Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
1.14.13.195 additional information when the enzyme is reduced with NADPH and reacts with molecular oxygen, a C4a-hydroperoxyflavin intermediate is observed. When the enzyme is reduced with NADH, the intermediate is 2fold less stable. Steady-state kinetic isotope effect values for NADPH and NADH are 3 and 2 , respectively, due to differences in the rate of flavin reduction by these coenzymes. NADP+, and not NAD+, protects the enzyme from proteolysis, suggesting that it induces conformational changes upon binding Aspergillus fumigatus ?
-
 ?
1.14.13.196 L-ornithine + NADH + H+ + O2 first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
1.14.13.196 L-ornithine + NADH + H+ + O2 accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme Aspergillus fumigatus N5-hydroxy-L-ornithine + NAD+ + H2O
-
 ?
1.14.13.196 L-ornithine + NADPH + H+ + O2 first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
1.14.13.196 L-ornithine + NADPH + H+ + O2 accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme Aspergillus fumigatus N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.195 SidA
-
 Aspergillus fumigatus
1.14.13.195 siderophore A
-
 Aspergillus fumigatus
1.14.13.196 SidA
-
 Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.195 0.6
-
 NADPH pH 7.5, 15°C Aspergillus fumigatus
1.14.13.195 0.73
-
 NADH pH 7.5, 15°C Aspergillus fumigatus
1.14.13.196 0.6
-
 NADPH pH 7.5, temperature not specified in the publication Aspergillus fumigatus
1.14.13.196 0.73
-
 NADH pH 7.5, temperature not specified in the publication Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.196 NADH accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme Aspergillus fumigatus
1.14.13.196 NADPH accepts reducing equivalents from either NADPH or NADH and displays similar kinetic parameters when using either coenzyme. NADP+, and not NAD+, protects the enzyme from proteolysis Aspergillus fumigatus

General Information

EC Number General Information Comment Organism
1.14.13.195 physiological function enzyme is essential for virulence Aspergillus fumigatus
1.14.13.196 physiological function first step in the biosynthesis of hydroxamate-containing siderophores, essential for virulence Aspergillus fumigatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.195 10
-
 NADH pH 7.5, 15°C Aspergillus fumigatus
1.14.13.195 50
-
 NADPH pH 7.5, 15°C Aspergillus fumigatus
1.14.13.196 7.3
-
 NADH pH 7.5, temperature not specified in the publication Aspergillus fumigatus
1.14.13.196 50
-
 NADPH pH 7.5, temperature not specified in the publication Aspergillus fumigatus