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Literature summary extracted from

  • Gannavaram, S.; Gadda, G.
    Relative timing of hydrogen and proton transfers in the reaction of flavin oxidation catalyzed by choline oxidase (2013), Biochemistry, 52, 1221-1226.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.17 additional information
-
 additional information stopped-flow kinetics of oxidative half-reaction, deuterium kinetic isotope effects in steady-state kinetics, e.g.. second-order rate constants, overview Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.17 choline + 2 O2 + H2O Arthrobacter globiformis
-
 betaine + 2 H2O2
-
 ?
1.1.3.17 choline + 2 O2 + H2O Arthrobacter globiformis ATCC 8010
-
 betaine + 2 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Arthrobacter globiformis
-
-
-
1.1.3.17 Arthrobacter globiformis ATCC 8010
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.17 choline + 2 O2 + H2O = betaine + 2 H2O2 reaction mechanism of reductive and oxidative half-reactions, determination of a mechanism of flavin oxidation that directly results in the formation of oxidized flavin and hydrogen peroxide without stabilization of reaction intermediates, overview Arthrobacter globiformis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.17 commercial preparation
-
 Arthrobacter globiformis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 choline + 2 O2 + H2O
-
 Arthrobacter globiformis betaine + 2 H2O2
-
 ?
1.1.3.17 choline + 2 O2 + H2O
-
 Arthrobacter globiformis ATCC 8010 betaine + 2 H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.17 25
-
 assay at Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.17 7 9 assay at Arthrobacter globiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.17 6 10 kcat/Kox with choline or 1,2-[2H4]choline as a substrate for choline oxidase is independent of pH between pH 6.0 and pH 10.0 Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.17 FAD
-
 Arthrobacter globiformis