EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.3.6 | Strep-Tag-fusion proteins expressed in Escherichia coli BLR (DE3) | Plasmodium falciparum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.3.6 | D26A/K83A/K151A | no formation of an internal chromophore with characteristic absorbance at 320 nm during reaction (observed with wild type protein) | Plasmodium falciparum |
4.3.3.6 | E136A/R139A/R140A | formation of an internal chromophore with characteristic absorbance at 320 nm during reaction (also observed with wild type protein) | Plasmodium falciparum |
4.3.3.6 | additional information | multiple deletion mutant proteins | Plasmodium falciparum |
4.3.3.6 | R167A | reduced formation of an internal chromophore with characteristic absorbance at 320 nm during reaction (observed with wild type protein), 50% wild type activity | Plasmodium falciparum |
4.3.3.6 | R85A/H88A/E91A | no formation of an internal chromophore with characteristic absorbance at 320 nm during reaction (observed with wild type protein), dodecameric assembly prevented | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.3.6 | 2-deoxy-D-ribose 5-phosphate | at 12 mM 56% residual activity | Plasmodium falciparum | |
4.3.3.6 | 4-phospho-D-erythronhydrazide | - |
Plasmodium falciparum | |
4.3.3.6 | D-erythronhydrazide | - |
Plasmodium falciparum | |
4.3.3.6 | D-erythrose | - |
Plasmodium falciparum | |
4.3.3.6 | D-erythrose 4-phosphate | - |
Plasmodium falciparum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.6 | Plasmodium falciparum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.3.6 | affinity chromatography using the Strep-Tag | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.6 | D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+ | - |
Plasmodium falciparum | pyridoxal 5'-phosphate + 4 H2O + phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.6 | Pdx1 | pyridoxal 5'-phosphate synthase complex consits of Pdx1 and Pdx2 | Plasmodium falciparum |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
4.3.3.6 | 0.016 | - |
Pdx1 in the heteromeric pyridoxal 5'-phosphate synthase complex, pH 8.0, 37°C | Plasmodium falciparum | 4-phospho-D-erythronhydrazide | |
4.3.3.6 | 0.043 | - |
Pdx1, pH 8.0, 37°C | Plasmodium falciparum | 4-phospho-D-erythronhydrazide | |
4.3.3.6 | 0.902 | - |
Pdx1, pH 8.0, 37°C | Plasmodium falciparum | D-erythronhydrazide | |
4.3.3.6 | 3.7 | - |
Pdx1, pH 8.0, 37°C | Plasmodium falciparum | D-erythrose 4-phosphate | |
4.3.3.6 | 160 | - |
Pdx1, pH 8.0, 37°C | Plasmodium falciparum | D-erythrose |